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Citations to this article

The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice
Christie L. Bell, … , Mavis Agbandje-McKenna, James M. Wilson
Christie L. Bell, … , Mavis Agbandje-McKenna, James M. Wilson
Published May 16, 2011
Citation Information: J Clin Invest. 2011;121(6):2427-2435. https://doi.org/10.1172/JCI57367.
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Research Article Genetics Article has an altmetric score of 9

The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice

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Abstract

Vectors based on adeno-associated virus (AAV) serotype 9 are candidates for in vivo gene delivery to many organs, but the receptor(s) mediating these tropisms have yet to be defined. We evaluated AAV9 uptake by glycans with terminal sialic acids (SAs), a common mode of cellular entry for viruses. We found, however, that AAV9 binding increased when terminal SA was enzymatically removed, suggesting that galactose, which is the most commonly observed penultimate monosaccharide to SA, may mediate AAV9 transduction. This was confirmed in mutant CHO Pro-5 cells deficient in the enzymes involved in glycoprotein biogenesis, as well as lectin interference studies. Binding of AAV9 to glycans with terminal galactose was demonstrated via glycan binding assays. Co-instillation of AAV9 vector with neuraminidase into mouse lung resulted in exposure of terminal galactose on the apical surface of conducting airway epithelial cells, as shown by lectin binding and increased transduction of these cells, demonstrating the possible utility of this vector in lung-directed gene transfer. Increasing the abundance of the receptor on target cells and improving vector efficacy may improve delivery of AAV vectors to their therapeutic targets.

Authors

Christie L. Bell, Luk H. Vandenberghe, Peter Bell, Maria P. Limberis, Guang-Ping Gao, Kim Van Vliet, Mavis Agbandje-McKenna, James M. Wilson

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2009 Total
Citations: 5 8 10 11 9 6 6 11 2 6 7 15 16 11 1 124
Citation information
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Citations to this article in year 2020 (6)

Title and authors Publication Year
Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors
R Bates, W Huang, L Cao
Molecular Therapy — Methods & Clinical Development 2020
Recommendations for the Development of Cell-Based Anti-Viral Vector Neutralizing Antibody Assays
G Boris, M Fiscella, M Havert, E Koren, B Long, M Milton, S Purushothama
The AAPS Journal 2020
Positron emission tomography imaging of novel AAV capsids maps rapid brain accumulation
JW Seo, ES Ingham, L Mahakian, S Tumbale, B Wu, S Aghevlian, S Shams, M Baikoghli, P Jain, X Ding, N Goeden, T Dobreva, NC Flytzanis, M Chavez, K Singhal, R Leib, ML James, DJ Segal, RH Cheng, EA Silva, V Gradinaru, KW Ferrara
Nature Communications 2020
Corneal Regeneration: Methods and Protocols
M Ahearne
2020
An ace model for SARS-CoV-2 infection
J Major, A Wack
Journal of Experimental Medicine 2020
Of rAAV and Men: From Genetic Neuromuscular Disorder Efficacy and Toxicity Preclinical Studies to Clinical Trials and Back
L Buscara, DA Gross, N Daniele
Journal of Personalized Medicine 2020

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Referenced in 27 patents
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