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Citations to this article

The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice
Christie L. Bell, … , Mavis Agbandje-McKenna, James M. Wilson
Christie L. Bell, … , Mavis Agbandje-McKenna, James M. Wilson
Published May 16, 2011
Citation Information: J Clin Invest. 2011;121(6):2427-2435. https://doi.org/10.1172/JCI57367.
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Research Article Genetics Article has an altmetric score of 9

The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice

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Abstract

Vectors based on adeno-associated virus (AAV) serotype 9 are candidates for in vivo gene delivery to many organs, but the receptor(s) mediating these tropisms have yet to be defined. We evaluated AAV9 uptake by glycans with terminal sialic acids (SAs), a common mode of cellular entry for viruses. We found, however, that AAV9 binding increased when terminal SA was enzymatically removed, suggesting that galactose, which is the most commonly observed penultimate monosaccharide to SA, may mediate AAV9 transduction. This was confirmed in mutant CHO Pro-5 cells deficient in the enzymes involved in glycoprotein biogenesis, as well as lectin interference studies. Binding of AAV9 to glycans with terminal galactose was demonstrated via glycan binding assays. Co-instillation of AAV9 vector with neuraminidase into mouse lung resulted in exposure of terminal galactose on the apical surface of conducting airway epithelial cells, as shown by lectin binding and increased transduction of these cells, demonstrating the possible utility of this vector in lung-directed gene transfer. Increasing the abundance of the receptor on target cells and improving vector efficacy may improve delivery of AAV vectors to their therapeutic targets.

Authors

Christie L. Bell, Luk H. Vandenberghe, Peter Bell, Maria P. Limberis, Guang-Ping Gao, Kim Van Vliet, Mavis Agbandje-McKenna, James M. Wilson

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2009 Total
Citations: 5 8 10 11 9 6 6 11 2 6 7 15 16 11 1 124
Citation information
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Citations to this article in year 2013 (16)

Title and authors Publication Year
Whole-body Correction of Mucopolysaccharidosis-IIIA by Intra-Cerebrospinal Fluid Gene Therapy
Virginia Haurigot, Sara Marcó, Albert Ribera, Miquel Garcia, Albert Ruzo, Pilar Villacampa, Eduardo Ayuso, Sonia Añor, Anna Andaluz, Mercedes Pineda, Gemma García Fructuoso, Maria Molas, Luca Maggioni, Sergio Muñoz, Sandra Motas, Jesus Ruberte, Federico Mingozzi, Marti Pumarola, Fàtima Bosch
Journal of Clinical Investigation 2013
AAV9 targets cone photoreceptors in the nonhuman primate retina
LH Vandenberghe, P Bell, AM Maguire, R Xiao, TB Hopkins, R Grant, J Bennett, JM Wilson
PloS one 2013
Postentry Processing of Recombinant Adeno-Associated Virus Type 1 and Transduction of the Ferret Lung Are Altered by a Factor in Airway Secretions
Z Yan, X Sun, IA Evans, SR Tyler, Y Song, X Liu, H Sui, JF Engelhardt
Human Gene Therapy 2013
Structural insights into adeno-associated virus serotype 5
L Govindasamy, MA DiMattia, BL Gurda, S Halder, R McKenna, JA Chiorini, N Muzyczka, S Zolotukhin, M Agbandje-McKenna
Journal of virology 2013
An Emerging Adeno-Associated Viral Vector Pipeline for Cardiac Gene Therapy
A Asokan, RJ Samulski
Human Gene Therapy 2013
Multiple Recombinant Adeno-Associated Viral Vector Serotypes Display Persistent In Vivo Gene Expression in Vector-Transduced Rat Stifle Joints
JB Mason, BL Gurda, JB Engiles, KD Hankenson, JM Wilson, DW Richardson
Human Gene Therapy Methods 2013
Replication of Respiratory Syncytial Virus Is Inhibited by the Host Defense Molecule Viperin
G McGillivary, ZB Jordan, ME Peeples, LO Bakaletz
Journal of Innate Immunity 2013
Intranasal Antibody Gene Transfer in Mice and Ferrets Elicits Broad Protection Against Pandemic Influenza
MP Limberis, VS Adam, G Wong, J Gren, D Kobasa, TM Ross, GP Kobinger, A Tretiakova, JM Wilson
Science Translational Medicine 2013
Carbohydrate microarrays
S Park, JC Gildersleeve, O Blixt, I Shin
Chemical Society Reviews 2013
Structure and dynamics of adeno-associated virus serotype 1 VP1-unique N-terminal domain and its role in capsid trafficking
B Venkatakrishnan, J Yarbrough, J Domsic, A Bennett, B Bothner, OG Kozyreva, RJ Samulski, N Muzyczka, R McKenna, M Agbandje-McKenna
Journal of virology 2013
Long-distance Axonal Transport of AAV9 Is Driven by Dynein and Kinesin-2 and Is Trafficked in a Highly Motile Rab7-positive Compartment
MJ Castle, E Perlson, EL Holzbaur, JH Wolfe
Molecular Therapy 2013
Efficient gene delivery and selective transduction of astrocytes in the mammalian brain using viral vectors
N Merienne, JL Douce, E Faivre, N Déglon, G Bonvento
2013
Engraftment of a Galactose Receptor Footprint onto Adeno-associated Viral Capsids Improves Transduction Efficiency
S Shen, ED Horowitz, AN Troupes, SM Brown, N Pulicherla, RJ Samulski, M Agbandje-McKenna, A Asokan
2013
Differential Adeno-Associated Virus Serotype-Specific Interaction Patterns with Synthetic Heparins and Other Glycans
M Mietzsch, F Broecker, A Reinhardt, PH Seeberger, R Heilbronn, MJ Imperiale
Journal of virology 2013
Comparative Analysis of Adeno-Associated Virus Capsid Stability and Dynamics
V Rayaprolu, S Kruse, R Kant, B Venkatakrishnan, N Movahed, D Brooke, B Lins, A Bennett, T Potter, R McKenna, M Agbandje-McKenna, B Bothner
Journal of virology 2013
Multiple Roles for Sialylated Glycans in Determining the Cardiopulmonary Tropism of Adeno-Associated Virus 4
S Shen, AN Troupes, N Pulicherla, A Asokan
Journal of virology 2013

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