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Citations to this article

The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice
Christie L. Bell, … , Mavis Agbandje-McKenna, James M. Wilson
Christie L. Bell, … , Mavis Agbandje-McKenna, James M. Wilson
Published May 16, 2011
Citation Information: J Clin Invest. 2011;121(6):2427-2435. https://doi.org/10.1172/JCI57367.
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Research Article Genetics Article has an altmetric score of 9

The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice

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Abstract

Vectors based on adeno-associated virus (AAV) serotype 9 are candidates for in vivo gene delivery to many organs, but the receptor(s) mediating these tropisms have yet to be defined. We evaluated AAV9 uptake by glycans with terminal sialic acids (SAs), a common mode of cellular entry for viruses. We found, however, that AAV9 binding increased when terminal SA was enzymatically removed, suggesting that galactose, which is the most commonly observed penultimate monosaccharide to SA, may mediate AAV9 transduction. This was confirmed in mutant CHO Pro-5 cells deficient in the enzymes involved in glycoprotein biogenesis, as well as lectin interference studies. Binding of AAV9 to glycans with terminal galactose was demonstrated via glycan binding assays. Co-instillation of AAV9 vector with neuraminidase into mouse lung resulted in exposure of terminal galactose on the apical surface of conducting airway epithelial cells, as shown by lectin binding and increased transduction of these cells, demonstrating the possible utility of this vector in lung-directed gene transfer. Increasing the abundance of the receptor on target cells and improving vector efficacy may improve delivery of AAV vectors to their therapeutic targets.

Authors

Christie L. Bell, Luk H. Vandenberghe, Peter Bell, Maria P. Limberis, Guang-Ping Gao, Kim Van Vliet, Mavis Agbandje-McKenna, James M. Wilson

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2009 Total
Citations: 5 8 10 11 9 6 6 11 2 6 7 15 16 11 1 124
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Citations to this article in year 2016 (6)

Title and authors Publication Year
CNS-restricted Transduction and CRISPR/Cas9-mediated Gene Deletion with an Engineered AAV Vector
G Murlidharan, K Sakamoto, L Rao, T Corriher, D Wang, G Gao, P Sullivan, A Asokan
Molecular Therapy — Nucleic Acids 2016
In Vivo Selection Yields AAV-B1 Capsid for Central Nervous System and Muscle Gene Therapy
SR Choudhury, Z Fitzpatrick, AF Harris, SA Maitland, JS Ferreira, Y Zhang, S Ma, RB Sharma, HL Gray-Edwards, JA Johnson, AK Johnson, LC Alonso, C Punzo, KR Wagner, CA Maguire, RM Kotin, DR Martin, M Sena-Esteves
Molecular Therapy 2016
Viral vectors for therapy of neurologic diseases
SR Choudhury, E Hudry, CA Maguire, M Sena-Esteves, XO Breakefield, P Grandi
Neuropharmacology 2016
Methods in Molecular Biology
MJ Castle, HT Turunen, LH Vandenberghe, JH Wolfe
Methods in molecular biology (Clifton, N.J.) 2016
Pulmonary ORMDL3 is critical for induction of Alternaria-induced allergic airways disease
S Löser, LG Gregory, Y Zhang, K Schaefer, SA Walker, J Buckley, L Denney, CH Dean, WO Cookson, MF Moffatt, CM Lloyd
Journal of Allergy and Clinical Immunology 2016
Trafficking of adeno-associated virus vectors across a model of the blood-brain barrier; a comparative study of transcytosis and transduction using primary human brain endothelial cells
SF Merkel, AM Andrews, EM Lutton, D Mu, E Hudry, BT Hyman, CA Maguire, SH Ramirez
Journal of Neurochemistry 2016

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Referenced in 27 patents
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