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Citations to this article

The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice
Christie L. Bell, … , Mavis Agbandje-McKenna, James M. Wilson
Christie L. Bell, … , Mavis Agbandje-McKenna, James M. Wilson
Published May 16, 2011
Citation Information: J Clin Invest. 2011;121(6):2427-2435. https://doi.org/10.1172/JCI57367.
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Research Article Genetics Article has an altmetric score of 9

The AAV9 receptor and its modification to improve in vivo lung gene transfer in mice

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Abstract

Vectors based on adeno-associated virus (AAV) serotype 9 are candidates for in vivo gene delivery to many organs, but the receptor(s) mediating these tropisms have yet to be defined. We evaluated AAV9 uptake by glycans with terminal sialic acids (SAs), a common mode of cellular entry for viruses. We found, however, that AAV9 binding increased when terminal SA was enzymatically removed, suggesting that galactose, which is the most commonly observed penultimate monosaccharide to SA, may mediate AAV9 transduction. This was confirmed in mutant CHO Pro-5 cells deficient in the enzymes involved in glycoprotein biogenesis, as well as lectin interference studies. Binding of AAV9 to glycans with terminal galactose was demonstrated via glycan binding assays. Co-instillation of AAV9 vector with neuraminidase into mouse lung resulted in exposure of terminal galactose on the apical surface of conducting airway epithelial cells, as shown by lectin binding and increased transduction of these cells, demonstrating the possible utility of this vector in lung-directed gene transfer. Increasing the abundance of the receptor on target cells and improving vector efficacy may improve delivery of AAV vectors to their therapeutic targets.

Authors

Christie L. Bell, Luk H. Vandenberghe, Peter Bell, Maria P. Limberis, Guang-Ping Gao, Kim Van Vliet, Mavis Agbandje-McKenna, James M. Wilson

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2009 Total
Citations: 5 8 10 11 9 6 6 11 2 6 7 15 16 11 1 124
Citation information
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Citations to this article in year 2014 (15)

Title and authors Publication Year
Naturally enveloped AAV vectors for shielding neutralizing antibodies and robust gene delivery in vivo
B György, Z Fitzpatrick, MH Crommentuijn, D Mu, CA Maguire
Biomaterials 2014
Systemic gene delivery to the central nervous system using Adeno-associated virus
M Bourdenx, N Dutheil, E Bezard, B Dehay
Frontiers in molecular neuroscience 2014
Drawing a high-resolution functional map of adeno-associated virus capsid by massively parallel sequencing
K Adachi, T Enoki, Y Kawano, M Veraz, H Nakai
Nature Communications 2014
Biodistribution of adeno-associated virus serotype 9 (AAV9) vector after intrathecal and intravenous delivery in mouse
DJ Schuster, JA Dykstra, MS Riedl, KF Kitto, LR Belur, RS McIvor, RP Elde, CA Fairbanks, L Vulchanova
Frontiers in neuroanatomy 2014
Adeno-Associated Virus Serotypes 1, 8, and 9 Share Conserved Mechanisms for Anterograde and Retrograde Axonal Transport
MJ Castle, ZT Gershenson, AR Giles, EL Holzbaur, JH Wolfe
Human Gene Therapy 2014
Intracisternal delivery of AAV9 results in oligodendrocyte and motor neuron transduction in the whole central nervous system of cats
T Bucher, L Dubreil, MA Colle, M Maquigneau, J Deniaud, M Ledevin, P Moullier, B Joussemet
Gene Therapy 2014
Gene Transfer Properties and Structural Modeling of Human Stem Cell-Derived AAV
LJ Smith, T Ul-Hasan, SK Carvaines, KV Vliet, E Yang, KK Wong, M Agbandje-McKenna, S Chatterjee
Molecular Therapy 2014
Adeno-associated virus: fit to serve
E Zinn, LH Vandenberghe
Current Opinion in Virology 2014
In vivo evaluation of adeno-associated virus gene transfer in airways of mice with acute or chronic respiratory infection
M Myint, M Limberis, P Bell, S Somanathan, A Haczku, JM Wilson, SL Diamond
Human Gene Therapy 2014
The Expression Pattern of Systemically Injected AAV9 in the Developing Mouse Retina Is Determined by Age
LC Byrne, YJ Lin, T Lee, DV Schaffer, JG Flannery
Molecular Therapy 2014
Biology of adeno-associated viral vectors in the central nervous system
G Murlidharan, RJ Samulski, A Asokan
Frontiers in molecular neuroscience 2014
CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
RJ Platt, S Chen, Y Zhou, MJ Yim, L Swiech, HR Kempton, JE Dahlman, O Parnas, TM Eisenhaure, M Jovanovic, DB Graham, S Jhunjhunwala, M Heidenreich, RJ Xavier, R Langer, DG Anderson, N Hacohen, A Regev, G Feng, PA Sharp, F Zhang
Cell 2014
Intravenous AAV9 efficiently transduces myenteric neurons in neonate and juvenile mice
SE Gombash, CJ Cowley, JA Fitzgerald, JC Hall, C Mueller, FL Christofi, KD Foust
Frontiers in molecular neuroscience 2014
Profiling of Glycan Receptors for Minute Virus of Mice in Permissive Cell Lines Towards Understanding the Mechanism of Cell Recognition
S Halder, S Cotmore, J Heimburg-Molinaro, DF Smith, RD Cummings, X Chen, AJ Trollope, SJ North, SM Haslam, A Dell, P Tattersall, R McKenna, M Agbandje-McKenna, S Pöhlmann
PloS one 2014
Functional Analysis of the Putative Integrin Recognition Motif on Adeno-associated Virus 9
S Shen, GE Berry, RM Rivera, RY Cheung, AN Troupes, SM Brown, T Kafri, A Asokan
The Journal of biological chemistry 2014

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