Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Pancreatic Cancer (Jul 2025)
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact

Citations to this article

Human lupus autoantibody–DNA complexes activate DCs through cooperation of CD32 and TLR9
Terry K. Means, … , Douglas T. Golenbock, Andrew D. Luster
Terry K. Means, … , Douglas T. Golenbock, Andrew D. Luster
Published February 1, 2005
Citation Information: J Clin Invest. 2005;115(2):407-417. https://doi.org/10.1172/JCI23025.
View: Text | PDF
Article Autoimmunity Article has an altmetric score of 14

Human lupus autoantibody–DNA complexes activate DCs through cooperation of CD32 and TLR9

  • Text
  • PDF
Abstract

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by pathogenic autoantibodies against nucleoproteins and DNA. Here we show that DNA-containing immune complexes (ICs) within lupus serum (SLE-ICs), but not protein-containing ICs from other autoimmune rheumatic diseases, stimulates plasmacytoid DCs (PDCs) to produce cytokines and chemokines via a cooperative interaction between Toll-like receptor 9 (TLR9) and FcγRIIa (CD32). SLE-ICs transiently colocalized to a subcellular compartment containing CD32 and TLR9, and CD32+, but not CD32–, PDCs internalized and responded to SLE-ICs. Our findings demonstrate a novel functional interaction between Fc receptors and TLRs, defining a pathway in which CD32 delivers SLE-ICs to intracellular lysosomes containing TLR9, inducing a signaling cascade leading to PDC activation. These data demonstrate that endogenous DNA-containing autoantibody complexes found in the serum of patients with SLE activate the innate immune system and suggest a novel mechanism whereby these ICs contribute to the pathogenesis of this autoimmune disease.

Authors

Terry K. Means, Eicke Latz, Fumitaka Hayashi, Mandakolathur R. Murali, Douglas T. Golenbock, Andrew D. Luster

×

Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Total
Citations: 3 14 10 13 16 9 21 18 17 14 22 21 17 24 18 26 22 13 6 7 11 322
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2023 (10)

Title and authors Publication Year
NLRP12: an innate immune checkpoint represses IFN signatures and ameliorates the progression of lupus nephritis
Yen-Po Tsao, Fang-Yu Tseng, Chih-Wei Chao, Ming-Han Chen, Pei-Ching Chang, Yi-Chen Yeh, Babamale Olarewaju Abdulkareem, Pin-Hsuan Wang, Wen-Ting Chuang, Chien-Sheng Wu, Chang-Youh Tsai, Szu-Ting Chen
Journal of Clinical Investigation 2023
Targeted Small Molecules for Systemic Lupus Erythematosus: Drugs in the Pipeline.
Mok CC
Drugs 2023
Disruption of endosomal trafficking with EGA alters TLR9 cytokine response in human plasmacytoid dendritic cells
Wiest MJ, Gu C, Ham H, Gorvel L, Keddis MT, Griffing LW, Joo H, Gorvel JP, Billadeau DD, Oh S
Frontiers in immunology 2023
Cutaneous Lupus Erythematosus: An Update on Pathogenesis and Future Therapeutic Directions.
Niebel D, de Vos L, Fetter T, Brägelmann C, Wenzel J
American Journal of Clinical Dermatology 2023
B cell intrinsic TLR7 expression drives severe lupus in TLR9 deficient mice
Haylee Cosgrove, Sebastien Gingras, Minjung Kim, Sheldon Bastacky, Jeremy Tilstra, Mark Jay Shlomchik
JCI Insight 2023
CSL362 potently and specifically depletes pDCs invitro and ablates SLE-immune complex-induced IFN responses.
Monaghan KA, Hoi A, Gamell C, Tai TY, Linggi B, Jordan J, Cesaroni M, Sato T, Ng M, Oon S, Benson J, Wicks I, Morand E, Wilson N
iScience 2023
A Comprehensive Literature Review on Managing Systemic Lupus Erythematosus: Addressing Cardiovascular Disease Risk in Females and Its Autoimmune Disease Associations
Dar S, Koirala S, Khan A, Bellary MD, Patel AV, Mathew B, Singh R, Baigam N, Razzaq W, Abdin ZU, Khawaja UA
Cureus 2023
Lupus IgA1 autoantibodies synergize with IgG to enhance plasmacytoid dendritic cell responses to RNA-containing immune complexes
Waterman HR, Dufort MJ, Posso SE, Ni M, Li LZ, Zhu C, Raj P, Buckner JH, Hamerman JA
2023
PLASMACYTOID DENDRITIC CELLS: A DENDRITIC CELL IN DISGUISE
Hornero RA, Idoyaga J
Molecular Immunology 2023
TLR9 regulates the autophagy–lysosome pathway to promote dendritic cell maturation and activation by activating the TRAF6‐cGAS‐STING pathway
Liu Y, Wei F, Zhan Y, Wang R, Mo B, Lin S
The Kaohsiung Journal of Medical Sciences 2023

Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts

Blogged by 1
Referenced in 25 patents
232 readers on Mendeley
1 readers on CiteULike
See more details