Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Pancreatic Cancer (Jul 2025)
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact

Citations to this article

Human lupus autoantibody–DNA complexes activate DCs through cooperation of CD32 and TLR9
Terry K. Means, … , Douglas T. Golenbock, Andrew D. Luster
Terry K. Means, … , Douglas T. Golenbock, Andrew D. Luster
Published February 1, 2005
Citation Information: J Clin Invest. 2005;115(2):407-417. https://doi.org/10.1172/JCI23025.
View: Text | PDF
Article Autoimmunity Article has an altmetric score of 14

Human lupus autoantibody–DNA complexes activate DCs through cooperation of CD32 and TLR9

  • Text
  • PDF
Abstract

Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by pathogenic autoantibodies against nucleoproteins and DNA. Here we show that DNA-containing immune complexes (ICs) within lupus serum (SLE-ICs), but not protein-containing ICs from other autoimmune rheumatic diseases, stimulates plasmacytoid DCs (PDCs) to produce cytokines and chemokines via a cooperative interaction between Toll-like receptor 9 (TLR9) and FcγRIIa (CD32). SLE-ICs transiently colocalized to a subcellular compartment containing CD32 and TLR9, and CD32+, but not CD32–, PDCs internalized and responded to SLE-ICs. Our findings demonstrate a novel functional interaction between Fc receptors and TLRs, defining a pathway in which CD32 delivers SLE-ICs to intracellular lysosomes containing TLR9, inducing a signaling cascade leading to PDC activation. These data demonstrate that endogenous DNA-containing autoantibody complexes found in the serum of patients with SLE activate the innate immune system and suggest a novel mechanism whereby these ICs contribute to the pathogenesis of this autoimmune disease.

Authors

Terry K. Means, Eicke Latz, Fumitaka Hayashi, Mandakolathur R. Murali, Douglas T. Golenbock, Andrew D. Luster

×

Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 Total
Citations: 3 14 10 13 16 9 21 18 17 14 22 21 17 24 18 26 22 13 6 7 11 322
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2021 (16)

Title and authors Publication Year
Aim2 Couples With Ube2i for Sumoylation‐Mediated Repression of Interferon Signatures in Systemic Lupus Erythematosus
A Lu, S Wu, J Niu, M Cui, M Chen, WL Clapp, BJ Barnes, G Meng
Arthritis & Rheumatology 2021
Mechanisms of Photosensitivity in Autoimmunity
SN Estadt, MP Maz, J Musai, JM Kahlenberg
Journal of Investigative Dermatology 2021
Erythroid mitochondrial retention triggers myeloid-dependent type I interferon in human SLE
S Caielli, J Cardenas, AA de Jesus, J Baisch, L Walters, JP Blanck, P Balasubramanian, C Stagnar, M Ohouo, S Hong, L Nassi, K Stewart, J Fuller, J Gu, JF Banchereau, T Wright, R Goldbach-Mansky, V Pascual
Cell 2021
Dendritic Cell Tumor Vaccination via Fc Gamma Receptor Targeting: Lessons Learned from Pre-Clinical and Translational Studies
EG Alcaide, S Krishnarajah, F Junker
Human vaccines 2021
Microvesicles in active lupus nephritis show Toll‐like receptor 9‐dependent co‐expression of galectin‐3 binding protein and double‐stranded DNA
NS Rasmussen, CT Nielsen, CH Nielsen, S Jacobsen
Clinical & Experimental Immunology 2021
Deoxyribonuclease 1-Mediated Clearance of Circulating Chromatin Prevents From Immune Cell Activation and Pro-inflammatory Cytokine Production, a Phenomenon Amplified by Low Trap1 Activity: Consequences for Systemic Lupus Erythematosus
J Felux, A Erbacher, M Breckler, R Hervé, D Lemeiter, HG Mannherz, M Napirei, HG Rammensee, P Decker
Frontiers in immunology 2021
Harnessing Tolerogenic Histone Peptide Epitopes From Nucleosomes for Selective Down-Regulation of Pathogenic Autoimmune Response in Lupus (Past, Present, and Future)
SK Datta
Frontiers in immunology 2021
Plasmacytoid Dendritic Cells as a New Therapeutic Target for Autoimmune Pancreatitis and IgG4-Related Disease
K Minaga, T Watanabe, A Hara, T Yoshikawa, K Kamata, M Kudo
Frontiers in immunology 2021
Physiological and Pathological Inflammation Induced by Antibodies and Pentraxins
CE Geyer, L Mes, M Newling, J den Dunnen, W Hoepel
Cells 2021
To Trap a Pathogen: Neutrophil Extracellular Traps and Their Role in Mucosal Epithelial and Skin Diseases
C Domínguez-Díaz, GU Varela-Trinidad, G Muñoz-Sánchez, K Solórzano-Castanedo, KE Avila-Arrezola, L Iñiguez-Gutiérrez, V Delgado-Rizo, M Fafutis-Morris
Cells 2021
Toll-Like Receptors Gene Polymorphisms in Autoimmune Disease
Y Zhang, J Liu, C Wang, J Liu, W Lu
Frontiers in immunology 2021
The effect of an electronic medical record intervention on hydroxychloroquine prescribing habits and surveyed providers’ opinions of the 2016 American Academy of Ophthalmology guidelines in the rheumatology and dermatology practices of an academic institutionle
Overbury RS, Stoddard GJ, Pupaibool J, Hansen CB, Lebiedz-Odrobina D
BMC health services research 2021
Overcoming PD-1 Blockade Resistance with CpG-A Toll-Like Receptor 9 Agonist Vidutolimod in Patients with Metastatic Melanoma.
Ribas A, Medina T, Kirkwood JM, Zakharia Y, Gonzalez R, Davar D, Chmielowski B, Campbell KM, Bao R, Kelley H, Morris A, Mauro D, Wooldridge JE, Luke JJ, Weiner GJ, Krieg AM, Milhem MM
Cancer Discovery 2021
Serologic markers of Epstein-Barr virus reactivation are associated with increased disease activity, inflammation, and interferon pathway activation in patients with systemic lupus erythematosus
Wood RA, Guthridge L, Thurmond E, Guthridge CJ, Kheir JM, Bourn RL, Wagner CA, Chen H, DeJager W, Macwana SR, Kamp S, Lu R, Arriens C, Chakravarty EF, Thanou A, Merrill JT, Guthridge JM, James JA
2021
Systematic identification of genomic elements that regulate FCGR2A expression and harbor variants linked with autoimmune disease
Dahlqvist J, Fulco CP, Ray JP, Liechti T, de Boer CG, Lieb DJ, Eisenhaure TM, Engreitz JM, Roederer M, Hacohen N
Human Molecular Genetics 2021
Utilizing type I interferon expression in the identification of antiphospholipid syndrome subsets
Cecchi I, Radin M, Rodríguez-Carrio J, Tambralli A, Knight JS, Sciascia S
Expert Review of Clinical Immunology 2021

Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts

Blogged by 1
Referenced in 25 patents
232 readers on Mendeley
1 readers on CiteULike
See more details