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Citations to this article

The type 2 iodothyronine deiodinase is essential for adaptive thermogenesis in brown adipose tissue
Lucia A. de Jesus, … , P. Reed Larsen, Antonio C. Bianco
Lucia A. de Jesus, … , P. Reed Larsen, Antonio C. Bianco
Published November 1, 2001
Citation Information: J Clin Invest. 2001;108(9):1379-1385. https://doi.org/10.1172/JCI13803.
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The type 2 iodothyronine deiodinase is essential for adaptive thermogenesis in brown adipose tissue

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Abstract

Type 2 iodothyronine deiodinase (D2) is a selenoenzyme, the product of the recently cloned cAMP-dependent Dio2 gene, which increases 10- to 50-fold during cold stress only in brown adipose tissue (BAT). Here we report that despite a normal plasma 3,5,3′-triiodothyronine (T3) concentration, cold-exposed mice with targeted disruption of the Dio2 gene (Dio2–/–) become hypothermic due to impaired BAT thermogenesis and survive by compensatory shivering with consequent acute weight loss. This occurs despite normal basal mitochondrial uncoupling protein 1 (UCP1) concentration. In Dio2–/– brown adipocytes, the acute norepinephrine-, CL316,243-, or forskolin-induced increases in lipolysis, UCP1 mRNA, and O2 consumption are all reduced due to impaired cAMP generation. These hypothyroid-like abnormalities are completely reversed by a single injection of T3 14 hours earlier. Recent studies suggest that UCP1 is primarily dependent on thyroid hormone receptor β (TRβ) while the normal sympathetic response of brown adipocytes requires TRα. Intracellularly generated T3 may be required to saturate the TRα, which has an approximately fourfold lower T3-binding affinity than does TRβ. Thus, D2 is an essential component in the thyroid-sympathetic synergism required for thermal homeostasis in small mammals.

Authors

Lucia A. de Jesus, Suzy D. Carvalho, Mirian O. Ribeiro, Mark Schneider, Sung-Woo Kim, John W. Harney, P. Reed Larsen, Antonio C. Bianco

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2004 2003 Total
Citations: 2 11 15 18 26 11 15 20 7 18 15 18 10 7 20 6 12 7 5 1 1 1 246
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Citations to this article in year 2018 (20)

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Thyroid Hormone Nuclear Receptor: Methods and Protocols
M Plateroti, J Samarut
2018
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Changes in Body Compositions and Basal Metabolic Rates during Treatment of Graves’ Disease
MJ Kim, SW Cho, S Choi, DL Ju, DJ Park, YJ Park
International Journal of Endocrinology 2018
Similarities and Differences in the Peripheral Actions of Thyroid Hormones and Their Metabolites
RA Louzada, DP Carvalho
Frontiers in Endocrinology 2018
TRH Neurons and Thyroid Hormone Coordinate the Hypothalamic Response to Cold
Z Zhang, A Boelen, A Kalsbeek, E Fliers
European Thyroid Journal 2018
Natriuretic peptides promote glucose uptake in a cGMP-dependent manner in human adipocytes
M Coué, V Barquissau, P Morigny, K Louche, C Lefort, A Mairal, C Carpéné, N Viguerie, P Arner, D Langin, M Rydén, C Moro
Scientific Reports 2018
Evaluation and optimization of differentiation conditions for human primary brown adipocytes
XY Wang, LH You, XW Cui, Y Li, X Wang, PF Xu, LJ Zhu, J Wen, LX Pang, XR Guo, CB Ji
Scientific Reports 2018
Central regulation of energy metabolism by estrogens
Y Xu, M López
Molecular Metabolism 2018
Do TSH, FT3, and FT4 Impact BAT Visualization of Clinical FDG-PET/CT Images?
R Nishii, S Nagamachi, Y Mizutani, T Terada, S Kiyohara, H Wakamatsu, S Fujita, T Higashi, K Yoshinaga, T Saga, T Hirai
Contrast Media & Molecular Imaging 2018
Metabolic Effects of the Intracellular Regulation of Thyroid Hormone: Old Players, New Concepts
AG Cicatiello, DD Girolamo, M Dentice
Frontiers in Endocrinology 2018
Low temperature exposure induces browning of bone marrow stem cell derived adipocytes in vitro
K Velickovic, HA Leija, I Bloor, J Law, H Sacks, M Symonds, V Sottile
Scientific Reports 2018
Adipocyte DIO2 Expression Increases in Human Obesity but Is Not Related to Systemic Insulin Sensitivity
D Bradley, J Liu, A Blaszczak, V Wright, A Jalilvand, B Needleman, S Noria, D Renton, W Hsueh
Journal of Diabetes Research 2018
FGF21 decreases body weight without reducing food intake or bone mineral density in high-fat fed obese rhesus macaque monkeys
B Andersen, EM Straarup, KM Heppner, DL Takahashi, V Raffaele, GA Dissen, K Lewandowski, TB Bödvarsdottir, K Raun, KL Grove, P Kievit
International Journal of Obesity 2018
NT-PGC-1α deficiency decreases mitochondrial FA oxidation in brown adipose tissue and alters substrate utilization in vivo
J Kim, MS Park, K Ha, C Park, J Lee, RL Mynatt, JS Chang
Journal of lipid research 2018
Effect of Iodothyronines on Thermogenesis: Focus on Brown Adipose Tissue
F Cioffi, A Gentile, E Silvestri, F Goglia, A Lombardi
Frontiers in Endocrinology 2018
Promotion of lipid storage rather than of thermogenic competence by fetal versus newborn calf serum in primary cultures of brown adipocytes
JM de Jong, B Cannon, J Nedergaard
Adipocyte 2018
Lipoprotein Lipase Expression in Hypothalamus Is Involved in the Central Regulation of Thermogenesis and the Response to Cold Exposure
E Laperrousaz, RG Denis, N Kassis, C Contreras, M López, S Luquet, C Cruciani-Guglielmacci, C Magnan
Frontiers in Endocrinology 2018
Deficiency of type 2 iodothyronine deiodinase reduces necroptosis activity and oxidative stress responses in retinas of Leber congenital amaurosis model mice
F Yang, H Ma, MR Butler,
The FASEB Journal 2018
Deficiency in interleukin-18 promotes differentiation of brown adipose tissue resulting in fat accumulation despite dyslipidemia
K Yamanishi, S Maeda, S Kuwahara-Otani, T Hashimoto, K Ikubo, K Mukai, K Nakasho, N Gamachi, Y El-Darawish, W Li, D Okuzaki, Y Watanabe, H Yamanishi, H Okamura, H Matsunaga
Journal of Translational Medicine 2018
Thyroid hormone (T 3 ) stimulates brown adipose tissue activation via mitochondrial biogenesis and MTOR-mediated mitophagy
WW Yau, BK Singh, R Lesmana, J Zhou, RA Sinha, KA Wong, Y Wu, BH Bay, S Sugii, L Sun, PM Yen
Autophagy 2018

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