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Citations to this article

GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice.
M R Stämpfli, … , Z Xing, M Jordana
M R Stämpfli, … , Z Xing, M Jordana
Published November 1, 1998
Citation Information: J Clin Invest. 1998;102(9):1704-1714. https://doi.org/10.1172/JCI4160.
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Research Article Article has an altmetric score of 4

GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice.

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Abstract

The purpose of this study was to explore whether repeated exposure to aerosolized ovalbumin (OVA) in the context of local expression of GM-CSF can initiate a Th2-driven, eosinophilic inflammation in the airways. On day -1, Balb/c mice were infected intranasally with an adenovirus construct expressing GM-CSF (Ad/GM-CSF). From day 0 to day 9 mice were exposed daily to an OVA aerosol. Mice exposed to OVA alone did not show any evidence of airway inflammation. Mice receiving both Ad/GM-CSF and aerosolized OVA exhibited marked airway inflammation characterized by eosinophilia and goblet cell hyperplasia. Migration of eosinophils into the airway was preceded by a rise in IL-5 and IL-4. Both IL-5 and class II MHC were critically required to generate airway eosinophilia. After resolution, airway eosinophilia was reconstituted after a single OVA exposure. Flow cytometric analysis of dispersed lung cells revealed an increase in macrophages and dendritic cells expressing B7.1 and B7.2, and expansion of activated (CD69-expressing) CD4 and CD8 T cells in mice exposed to OVA and Ad/GM-CSF. Our data indicate that expression of GM-CSF in the airway compartment increases local antigen presentation capacity, and concomitantly facilitates the development of an antigen-specific, eosinophilic inflammatory response to an otherwise innocuous antigen.

Authors

M R Stämpfli, R E Wiley, G S Neigh, B U Gajewska, X F Lei, D P Snider, Z Xing, M Jordana

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Total citations by year

Year: 2025 2024 2023 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 Total
Citations: 1 4 1 2 1 1 2 2 5 3 7 11 12 10 11 12 14 8 12 16 9 9 19 16 9 7 204
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Citations to this article in year 2014 (7)

Title and authors Publication Year
GM-CSF-Licensed CD11b+ Lung Dendritic Cells Orchestrate Th2 Immunity to Blomia tropicalis
Q Zhou, AW Ho, A Schlitzer, Y Tang, KH Wong, FH Wong, YL Chua, V Angeli, A Mortellaro, F Ginhoux, DM Kemeny
Journal of immunology (Baltimore, Md. : 1950) 2014
Allergen Ligands in the Initiation of Allergic Sensitization
WR Thomas
Current Allergy and Asthma Reports 2014
Alveolar macrophages are sentinels of murine pulmonary homeostasis following inhaled antigen challenge
SA Mathie, KL Dixon, SA Walker, V Tyrrell, M Mondhe, VB O'Donnell, LG Gregory, CM Lloyd
Allergy 2014
Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis
GF Weber, BG Chousterman, I Hilgendorf, CS Robbins, I Theurl, LM Gerhardt, Y Iwamoto, TD Quach, M Ali, JW Chen, TL Rothstein, M Nahrendorf, R Weissleder, FK Swirski
Journal of Experimental Medicine 2014
A GM-CSF/IL-33 pathway facilitates allergic airway responses to sub-threshold house dust mite exposure
A Llop-Guevara, DK Chu, TD Walker, S Goncharova, R Fattouh, JS Silver, CL Moore, JL Xie, PM O'Byrne, AJ Coyle, R Kolbeck, AA Humbles, MR Stämpfli, M Jordana
PloS one 2014
A GM-CSF/IL-33 Pathway Facilitates Allergic Airway Responses to Sub-Threshold House Dust Mite Exposure
A Llop-Guevara, DK Chu, TD Walker, S Goncharova, R Fattouh, JS Silver, CL Moore, JL Xie, PM O’Byrne, AJ Coyle, R Kolbeck, AA Humbles, MR Stämpfli, M Jordana, HW Chu
PloS one 2014
Molecular mechanisms and physiology of disease
N Maulik, T Karagiannis
2014

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