Go to JCI Insight
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact
  • Clinical Research and Public Health
  • Current issue
  • Past issues
  • By specialty
    • COVID-19
    • Cardiology
    • Gastroenterology
    • Immunology
    • Metabolism
    • Nephrology
    • Neuroscience
    • Oncology
    • Pulmonology
    • Vascular biology
    • All ...
  • Videos
    • Conversations with Giants in Medicine
    • Video Abstracts
  • Reviews
    • View all reviews ...
    • Complement Biology and Therapeutics (May 2025)
    • Evolving insights into MASLD and MASH pathogenesis and treatment (Apr 2025)
    • Microbiome in Health and Disease (Feb 2025)
    • Substance Use Disorders (Oct 2024)
    • Clonal Hematopoiesis (Oct 2024)
    • Sex Differences in Medicine (Sep 2024)
    • Vascular Malformations (Apr 2024)
    • View all review series ...
  • Viewpoint
  • Collections
    • In-Press Preview
    • Clinical Research and Public Health
    • Research Letters
    • Letters to the Editor
    • Editorials
    • Commentaries
    • Editor's notes
    • Reviews
    • Viewpoints
    • 100th anniversary
    • Top read articles

  • Current issue
  • Past issues
  • Specialties
  • Reviews
  • Review series
  • Conversations with Giants in Medicine
  • Video Abstracts
  • In-Press Preview
  • Clinical Research and Public Health
  • Research Letters
  • Letters to the Editor
  • Editorials
  • Commentaries
  • Editor's notes
  • Reviews
  • Viewpoints
  • 100th anniversary
  • Top read articles
  • About
  • Editors
  • Consulting Editors
  • For authors
  • Publication ethics
  • Publication alerts by email
  • Advertising
  • Job board
  • Contact

Citations to this article

GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice.
M R Stämpfli, … , Z Xing, M Jordana
M R Stämpfli, … , Z Xing, M Jordana
Published November 1, 1998
Citation Information: J Clin Invest. 1998;102(9):1704-1714. https://doi.org/10.1172/JCI4160.
View: Text | PDF
Research Article Article has an altmetric score of 4

GM-CSF transgene expression in the airway allows aerosolized ovalbumin to induce allergic sensitization in mice.

  • Text
  • PDF
Abstract

The purpose of this study was to explore whether repeated exposure to aerosolized ovalbumin (OVA) in the context of local expression of GM-CSF can initiate a Th2-driven, eosinophilic inflammation in the airways. On day -1, Balb/c mice were infected intranasally with an adenovirus construct expressing GM-CSF (Ad/GM-CSF). From day 0 to day 9 mice were exposed daily to an OVA aerosol. Mice exposed to OVA alone did not show any evidence of airway inflammation. Mice receiving both Ad/GM-CSF and aerosolized OVA exhibited marked airway inflammation characterized by eosinophilia and goblet cell hyperplasia. Migration of eosinophils into the airway was preceded by a rise in IL-5 and IL-4. Both IL-5 and class II MHC were critically required to generate airway eosinophilia. After resolution, airway eosinophilia was reconstituted after a single OVA exposure. Flow cytometric analysis of dispersed lung cells revealed an increase in macrophages and dendritic cells expressing B7.1 and B7.2, and expansion of activated (CD69-expressing) CD4 and CD8 T cells in mice exposed to OVA and Ad/GM-CSF. Our data indicate that expression of GM-CSF in the airway compartment increases local antigen presentation capacity, and concomitantly facilitates the development of an antigen-specific, eosinophilic inflammatory response to an otherwise innocuous antigen.

Authors

M R Stämpfli, R E Wiley, G S Neigh, B U Gajewska, X F Lei, D P Snider, Z Xing, M Jordana

×

Total citations by year

Year: 2025 2024 2023 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 Total
Citations: 1 4 1 2 1 1 2 2 5 3 7 11 12 10 11 12 14 8 12 16 9 9 19 16 9 7 204
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2010 (11)

Title and authors Publication Year
Methods in Molecular Biology
P Pouliot, MA Willart, H Hammad, BN Lambrecht
Methods in molecular biology (Clifton, N.J.) 2010
Overexpression of Smad2 drives house dust mite-mediated airway remodeling and airway hyperresponsiveness via activin and IL-25
LG Gregory, SA Mathie, SA Walker, S Pegorier, CP Jones, CM Lloyd
American journal of respiratory and critical care medicine 2010
Progress in Understanding Postnatal Immune Dysregulation in Allergic Disease
SL Prescott, D Martino, M Hodder, T Richman, MK Tulic
World Allergy Organization Journal 2010
T-cell polarization depends on concentration of the danger signal used to activate dendritic cells
M Peters, K Dudziak, M Stiehm, A Bufe
Immunology and Cell Biology 2010
The role of dendritic and epithelial cells as master regulators of allergic airway inflammation
BN Lambrecht, H Hammad
The Lancet 2010
Alarming Dendritic Cells for Allergic Sensitization
MA Willart, H Hammad
Allergology International 2010
Airway Epithelial NF-κB Activation Promotes Allergic Sensitization to an Innocuous Inhaled Antigen
JL Ather, SR Hodgkins, YM Janssen-Heininger, ME Poynter
American journal of respiratory cell and molecular biology 2010
Improving Adenovirus Based Gene Transfer: Strategies to Accomplish Immune Evasion
SS Seregin, A Amalfitano
Viruses 2010
Neutralizing Granulocyte/Macrophage Colony–Stimulating Factor Inhibits Cigarette Smoke–induced Lung Inflammation
R Vlahos, S Bozinovski, SP Chan, S Ivanov, A Lindén, JA Hamilton, GP Anderson
American journal of respiratory and critical care medicine 2010
Rhodiola algida improves chemotherapy-induced oral mucositis in breast cancer patients
WT Loo, LJ Jin, LW Chow, MN Cheung, M Wang
Expert Opinion on Investigational Drugs 2010
Fas-Activated Serine/Threonine Phosphoprotein Promotes Immune-Mediated Pulmonary Inflammation
M Simarro, G Giannattasio, MA la Fuente, C Benarafa, KK Subramanian, R Ishizawar, B Balestrieri, EM Andersson, HR Luo, A Orduña, J Boyce, P Anderson
Journal of immunology (Baltimore, Md. : 1950) 2010

Advertisement

Copyright © 2025 American Society for Clinical Investigation
ISSN: 0021-9738 (print), 1558-8238 (online)

Sign up for email alerts

Referenced in 6 patents
Highlighted by 1 platforms
59 readers on Mendeley
See more details