(A) WB of UMOD in organoids. (B) Bright-field or double-IF of UMOD and BiP with DAPI in organoids. (C, F, and I) IB of indicated proteins in organoid-isolated TALs. (D) Luciferase activity in the organoid medium. (E) Transcript expression of ER calcium efflux channels in organoid-derived TALs. (G) IF of IP3R1 and UMOD. (H) Dual-IF staining of TRIB3 and UMOD. (J) Co-IP demonstrating the association of TRIB3 and AMPKα in TALs. (K–R) Organoids were treated without or with DOX from days 22–30. (K and N–P) IB of the indicated proteins in TALs. (L) Co-IP showing the interaction between MANF and IP3R1 in TALs with DOX. (M) Luciferase activity in the organoid medium. (Q) p-FLISA of cytokines in the organoid medium. (R) Heatmap of TAL healthy state markers. Benjamini-Hochberg adjusted P < 0.05. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.