(A) ATR knockdown decreases N-cadherin and increases E-cadherin levels. Lentivirus encoding control or shATR was used to infect MDA-MB-231 cells, and the cells were seeded in 6-well plates with different stiffnesses. E- and N-cadherin levels were then assessed. (B) Statistical analysis of the Western blot results in A was performed using 2-way ANOVA (n = 3). (C) MDA-MB-436 cells were infected with lentivirus encoding either control or shATR, and cells were seeded in 6-well plates and analyzed for E- and N-cadherin expression. (D) Statistical analysis of the Western blot results in C performed using 2-way ANOVA (n = 3). (E) ATR inhibition decreases N-cadherin and increases E-cadherin expression. MDA-MB-231 cells were treated with DMSO or VX970 (80 nM) and seeded on substrates of varying stiffness, after which E- and N-cadherin levels were assessed. (F) Statistical analysis of the Western blot results in E performed using 2-way ANOVA (n = 3). (G) MDA-MB-436 cells were treated with DMSO or VX970 (80 nM), seeded on substrates of varying stiffness, and subsequently harvested for analysis of E-cadherin and N-cadherin expression. (H) Statistical analysis of the Western blot results in G by 2-way ANOVA (n = 3). All statistical data represent biological replicates, and results are expressed as mean ± SD. Western blot and co-IP experiments were independently repeated at least 3 times with similar results.