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Research Article Free access | 10.1172/JCI116381

Expression of SV40 virus large T antigen by recombinant adenoviruses activates proliferation of corneal endothelium in vitro.

S T Feldman, R Gjerset, D Gately, K R Chien, and J R Feramisco

Department of Ophthalmology, University of California, San Diego, La Jolla 92093.

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Department of Ophthalmology, University of California, San Diego, La Jolla 92093.

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Department of Ophthalmology, University of California, San Diego, La Jolla 92093.

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Department of Ophthalmology, University of California, San Diego, La Jolla 92093.

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Department of Ophthalmology, University of California, San Diego, La Jolla 92093.

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Published April 1, 1993 - More info

Published in Volume 91, Issue 4 on April 1, 1993
J Clin Invest. 1993;91(4):1713–1720. https://doi.org/10.1172/JCI116381.
© 1993 The American Society for Clinical Investigation
Published April 1, 1993 - Version history
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Abstract

Infection with the Ad5-SVR4 virus was used to introduce the large T antigen encoding region of the SV40 virus into bovine and human corneal endothelial cells. Expression of large T antigen occurred in 40% of bovine corneal endothelial cells after a 24-h incubation time versus 12% after 8 h of incubation. By 48 h after infection, almost all (92.8%) bovine corneal endothelial cells expressed large T antigen. Bovine and human corneal endothelial cells which expressed large T antigen proliferated and the characteristic morphologic features of corneal endothelium were maintained. This method may enable growth of enough corneal endothelium to perform studies to elucidate the biochemical mechanisms involved in regulating endothelial cell function.

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