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Citations to this article

Defensins. Natural peptide antibiotics of human neutrophils.
T Ganz, … , D F Bainton, R I Lehrer
T Ganz, … , D F Bainton, R I Lehrer
Published October 1, 1985
Citation Information: J Clin Invest. 1985;76(4):1427-1435. https://doi.org/10.1172/JCI112120.
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Research Article Article has an altmetric score of 22

Defensins. Natural peptide antibiotics of human neutrophils.

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Abstract

We extracted a granule-rich sediment from normal human neutrophils and subjected it to chromatographic, electrophoretic, and functional analysis. The extract contained three small (molecular weight less than 3,500) antibiotic peptides that were named human neutrophil peptide (HNP)-1, HNP-2, and HNP-3, and which will be referred to as "defensins." HNP 1-3, a mixture of the three defensins, killed Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli effectively in vitro when tested in 10 mM phosphate buffer containing certain nutrients, but it had little or no bactericidal activity in nutrient-free buffer. In contrast, the nutrient-free buffer supported a high degree of activity by HNP 1-3 against Cryptococcus neoformans. In addition to its antibacterial and antifungal properties, HNP 1-3 directly inactivated herpes simplex virus, Type 1. Two of the individual purified defensins, HNP-1 and HNP-2, were as microbicidal as the mixture HNP 1-3. HNP-3 was less active than the other defensins against most but not all of the microbes tested. Immunoperoxidase stains revealed HNP 1-3 to have a granular localization in the neutrophil's cytoplasm by light microscopy. Frozen thin section immunogold transmission electron microscopy showed HNP 1-3 to be localized in azurophil granules. These studies define a broad-spectrum antimicrobial system in human neutrophils. The defensin system may operate in conjunction with or independently from oxygen-dependent microbicidal processes to enable human neutrophils to inactivate and destroy potential pathogens.

Authors

T Ganz, M E Selsted, D Szklarek, S S Harwig, K Daher, D F Bainton, R I Lehrer

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Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 1989 1988 1987 1986 1981 1979 Total
Citations: 7 15 20 21 33 26 29 30 26 38 36 38 29 26 34 40 34 43 40 29 38 37 35 27 24 23 36 16 17 16 15 16 18 26 32 32 32 28 10 7 1 1 1081
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Citations to this article in year 2017 (26)

Title and authors Publication Year
Update on Chlamydia trachomatis Vaccinology
LM de la Maza, G Zhong, RC Brunham, CJ Papasian
Clinical and vaccine immunology : CVI 2017
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BT Pace, AA Lackner, E Porter, B Pahar
Mediators of Inflammation 2017
Alarmins and immunity
D Yang, Z Han, JJ Oppenheim
Immunological Reviews 2017
Epithelial-mesenchymal transition and inflammation at the site of the primary tumor
C Dominguez, JM David, C Palena
Seminars in Cancer Biology 2017
HIV-Enhancing and HIV-Inhibiting Properties of Cationic Peptides and Proteins.
Cole AM, Cole AL
Viruses 2017
Snake Venoms
H Inagaki, CW Vogel, AK Mukherjee, TR Rahmy
Snake Venoms 2017
Synthetic approaches to radiochemical probes for imaging of bacterial infections
J Dutta, T Naicker, T Ebenhan, HG Kruger, PI Arvidsson, T Govender
European Journal of Medicinal Chemistry 2017
Clinical and Basic Immunodermatology
AA Gaspari, SK Tyring, DH Kaplan
Clinical and Basic Immunodermatology 2017
Friends or Foes? Host defense (antimicrobial) peptides and proteins in human skin diseases
F Niyonsaba, C Kiatsurayanon, P Chieosilapatham, H Ogawa
Experimental Dermatology 2017
Bioresources and Bioprocess in Biotechnology
S Sugathan, NS Pradeep, S Abdulhameed
Bioresources and Bioprocess in Biotechnology 2017
Targeting and inactivation of bacterial toxins by human defensins
E Kudryashova, SM Seveau, DS Kudryashov
Biological Chemistry 2017
Perspectives for clinical use of engineered human host defense antimicrobial peptides
ME Pachón-Ibáñez, Y Smani, J Pachón, J Sánchez-Céspedes
FEMS Microbiology Reviews 2017
Pathogenesis of Periodontal Diseases
N Bostanci, GN Belibasakis
Pathogenesis of Periodontal Diseases 2017
Defensins in Viral Infection and Pathogenesis
MK Holly, K Diaz, JG Smith
Annual Review of Virology 2017
Variations in the interaction of human defensins with Escherichia coli: Possible implications in bacterial killing
B Mathew, R Nagaraj, K Zeth
PloS one 2017
Comparative genomic identification and validation of β-defensin genes in the Ovis aries genome
TJ Hall, C McQuillan, EK Finlay, C OFarrelly, S Fair, KG Meade
BMC Genomics 2017
Ubiquitous Nature of Fluoroquinolones: The Oscillation between Antibacterial and Anticancer Activities
T Idowu, F Schweizer
Antibiotics 2017
Antimicrobial peptides as an alternative to anti-tuberculosis drugs
M AlMatar, EA Makky, G Yakıcı, I Var, B Kayar, F Köksal
Pharmacological Research 2017
Small Bioactive Peptides for Biomaterials Design and Therapeutics
IW Hamley
Chemical Reviews 2017
Surrogate Fecal Biomarkers in Inflammatory Bowel Disease: Rivals or Complementary Tools of Fecal Calprotectin?
MD Ruscio, F Vernia, A Ciccone, G Frieri, G Latella
Inflammatory Bowel Diseases 2017
Targeting a cell wall biosynthesis hot spot
A Müller, A Klöckner, T Schneider
Natural Product Reports 2017
Evolutionary Biology: Self/Nonself Evolution, Species and Complex Traits Evolution, Methods and Concepts
P Pontarotti
2017
Synovial Fluid α-Defensin as a Biomarker for Peri-Prosthetic Joint Infection: A Systematic Review and Meta-Analysis
B Li, F Chen, Y Liu, G Xu
Surgical Infections 2017
Expression of the zebrafish β-defensin 3 mature peptide in Pichia pastoris and its purification and antibacterial activity
Y Tao, CF Song, W Li
Applied Biochemistry and Microbiology 2017
Role of Streptococcus mutans two-component systems in antimicrobial peptide resistance in the oral cavity
M Kawada-Matsuo, H Komatsuzawa
The Japanese dental science review 2017
Associations between HBD3 and Pro-Inflammatory Cytokines in Asymptomatic Irreversible Pulpitis
A Aminoshariae, M Bakkar, T Bonfield, S Ghosh, TA Montagnese, AK Mickel
2017

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