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Citations to this article

Defensins. Natural peptide antibiotics of human neutrophils.
T Ganz, … , D F Bainton, R I Lehrer
T Ganz, … , D F Bainton, R I Lehrer
Published October 1, 1985
Citation Information: J Clin Invest. 1985;76(4):1427-1435. https://doi.org/10.1172/JCI112120.
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Research Article Article has an altmetric score of 22

Defensins. Natural peptide antibiotics of human neutrophils.

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Abstract

We extracted a granule-rich sediment from normal human neutrophils and subjected it to chromatographic, electrophoretic, and functional analysis. The extract contained three small (molecular weight less than 3,500) antibiotic peptides that were named human neutrophil peptide (HNP)-1, HNP-2, and HNP-3, and which will be referred to as "defensins." HNP 1-3, a mixture of the three defensins, killed Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli effectively in vitro when tested in 10 mM phosphate buffer containing certain nutrients, but it had little or no bactericidal activity in nutrient-free buffer. In contrast, the nutrient-free buffer supported a high degree of activity by HNP 1-3 against Cryptococcus neoformans. In addition to its antibacterial and antifungal properties, HNP 1-3 directly inactivated herpes simplex virus, Type 1. Two of the individual purified defensins, HNP-1 and HNP-2, were as microbicidal as the mixture HNP 1-3. HNP-3 was less active than the other defensins against most but not all of the microbes tested. Immunoperoxidase stains revealed HNP 1-3 to have a granular localization in the neutrophil's cytoplasm by light microscopy. Frozen thin section immunogold transmission electron microscopy showed HNP 1-3 to be localized in azurophil granules. These studies define a broad-spectrum antimicrobial system in human neutrophils. The defensin system may operate in conjunction with or independently from oxygen-dependent microbicidal processes to enable human neutrophils to inactivate and destroy potential pathogens.

Authors

T Ganz, M E Selsted, D Szklarek, S S Harwig, K Daher, D F Bainton, R I Lehrer

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Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1991 1990 1989 1988 1987 1986 1981 1979 Total
Citations: 7 15 20 21 33 26 29 30 26 38 36 38 29 26 34 40 34 43 40 29 38 37 35 27 24 23 36 16 17 16 15 16 18 26 32 32 32 28 10 7 1 1 1081
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Citations to this article in year 2020 (26)

Title and authors Publication Year
Innate Effector Systems in Primary Human Macrophages Sensitize Multidrug-Resistant Klebsiella pneumoniae to Antibiotics
RS Rekha, H Karadottir, S Ahmed, GH Gudmundsson, B Agerberth, P Bergman, M Raffatellu
Infection and immunity 2020
Antimicrobial host defence peptides: functions and clinical potential
N Mookherjee, MA Anderson, HP Haagsman, DJ Davidson
Nature Reviews Drug Discovery 2020
Topical antimicrobial peptide formulations for wound healing: Current developments and future prospects
RK Thapa, DB Diep, HH Tønnesen
Acta Biomaterialia 2020
Defensins: A Double-Edged Sword in Host Immunity
D Xu, W Lu
Frontiers in immunology 2020
Antiviral Activities of Human Host Defense Peptides
DC Brice, G Diamond
Current medicinal chemistry 2020
Human antimicrobial peptides in autoimmunity
ES Umnyakova, MS Zharkova, MN Berlov, OV Shamova, VN Kokryakov
Autoimmunity 2020
Cooperative Function of LL-37 and HNP1 Protects Mammalian Cell Membranes from Lysis
E Drab, K Sugihara
Biophysical Journal 2020
HNP-1 and HBD-1 as Biomarkers for the Immune Systems of Elite Basketball Athletes
R Pero, M Brancaccio, C Mennitti, L Gentile, A Franco, S Laneri, MD Biasi, C Pagliuca, R Colicchio, P Salvatore, G DAlicandro, D Terracciano, M Cennamo, EL Civita, A Liotti, C Mazzaccara, G Frisso, B Lombardo, O Scudiero
Antibiotics 2020
Enterotoxigenic Escherichia coli infection promotes enteric defensin expression via FOXO6-METTL3-m 6 A-GPR161 signalling axis
X Zong, H Wang, X Xiao, Y Zhang, Y Hu, F Wang, Y Wang, Z Lu
RNA biology 2020
Alpha-defensin lateral flow test does not appear to be useful in predicting shoulder periprosthetic joint infections
L Weigelt, A Plate, L Stadler, R Sutter, D Frustaci, R Zbinden, PO Zingg, C Gerber, Y Achermann
International Orthopaedics 2020
Anti-inflammatory therapy for COVID-19 infection: the case for colchicine
AZ Reyes, KA Hu, J Teperman, TL Muskardin, JC Tardif, B Shah, MH Pillinger
Annals of the rheumatic diseases 2020
Defensins: Transcriptional regulation and function beyond antimicrobial activity
G Contreras, I Shirdel, MS Braun, M Wink
Developmental and comparative immunology 2020
Synthetic Polypeptide Polymers as Simplified Analogues of Antimicrobial Peptides
P Salas-Ambrosio, A Tronnet, P Verhaeghe, C Bonduelle
Biomacromolecules 2020
Increased Synovial Inflammatory Markers in Aseptic Total Hip Arthroplasty Dislocation
HC Bäcker, S Hardt, JT Richards, C Perka, V Janz
The Journal of Arthroplasty 2020
Antimicrobial Peptides as Anticancer Agents: Functional Properties and Biological Activities
AL Tornesello, A Borrelli, L Buonaguro, FM Buonaguro, ML Tornesello
Molecules (Basel, Switzerland) 2020
Antibacterial activity of human defensins against Staphylococcus aureus and Escherichia coli
A Bolatchiev
PeerJ 2020
ExPortal and the LiaFSR Regulatory System Coordinate the Response to Cell Membrane Stress in Streptococcus pyogenes
Y Lin, MA Sanson, LA Vega, B Shah, S Regmi, MB Cubria, AR Flores, KA Kline
mBio 2020
Fragmentation of Human Neutrophil α-Defensin 4 to Combat Multidrug Resistant Bacteria
D Ehmann, L Koeninger, J Wendler, NP Malek, EF Stange, J Wehkamp, BA Jensen
Frontiers in microbiology 2020
Antibiofilm Activity on Candida albicans and Mechanism of Action on Biomembrane Models of the Antimicrobial Peptide Ctn[15–34]
FL de Aguiar, NC Santos, CS de Paula Cavalcante, D Andreu, GR Baptista, S Gonçalves
International journal of molecular sciences 2020
Mass Spectrometry-Based Identification of Urinary Antimicrobial Peptides in Dairy Cows
A Sharma, R Nigam, A Kumar, S Singh
Protein and peptide letters 2020
Antibiotics: A Bibliometric Analysis of Top 100 Classics
AI Arshad, P Ahmad, MI Karobari, JA Asif, MK Alam, Z Mahmood, NA Rahman, N Mamat, MA Kamal
Antibiotics 2020
A Crosstalk on Antimicrobial Peptides
A Borah, B Deb, S Chakraborty
International Journal of Peptide Research and Therapeutics 2020
Identification and characterization of urinary peptides using Q-TOF mass spectrometry in cow
A Sharma, R Nigam, A Kumar, A Pal
2020
Human Antimicrobial Peptides: Spectrum, Mode of Action and Resistance Mechanisms
BS Bazzaz, S Seyedi, NH Goki, B Khameneh
International Journal of Peptide Research and Therapeutics 2020
Antimicrobial Peptides: An Approach to Combat Resilient Infections
D Parai, P Dey, SK Mukherjee
Current drug discovery technologies 2020
Antibiotics: A Bibliometric Analysis of Top 100 Classics.
Arshad AI, Ahmad P, Karobari MI, Asif JA, Alam MK, Mahmood Z, Abd Rahman N, Mamat N, Kamal MA
2020

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