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Concise Publication Free access | 10.1172/JCI107427

Uric Acid: CHARACTERIZATION OF ITS INTERACTION WITH HUMAN SERUM ALBUMIN

David S. Campion, Rodney Bluestone, and James R. Klinenberg

Department of Medicine, UCLA School of Medicine, Los Angeles, California 90024

Wadsworth Veterans Administration Hospital, Los Angeles, California 90073

Divisions of Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90029

Find articles by Campion, D. in: JCI | PubMed | Google Scholar

Department of Medicine, UCLA School of Medicine, Los Angeles, California 90024

Wadsworth Veterans Administration Hospital, Los Angeles, California 90073

Divisions of Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90029

Find articles by Bluestone, R. in: JCI | PubMed | Google Scholar

Department of Medicine, UCLA School of Medicine, Los Angeles, California 90024

Wadsworth Veterans Administration Hospital, Los Angeles, California 90073

Divisions of Medicine, Cedars-Sinai Medical Center, Los Angeles, California 90029

Find articles by Klinenberg, J. in: JCI | PubMed | Google Scholar

Published September 1, 1973 - More info

Published in Volume 52, Issue 9 on September 1, 1973
J Clin Invest. 1973;52(9):2383–2386. https://doi.org/10.1172/JCI107427.
© 1973 The American Society for Clinical Investigation
Published September 1, 1973 - Version history
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Abstract

Binding of sodium urate to human serum albumin (HSA) was measured by continuous ultrafiltration at pH 7.4 and ionic strength 0.16 over the concentration range 1-13 mg/100 ml. The percent sodium urate bound to 5 g/100 ml HSA was constant over this concentration range: 30.3 (SE±0.6)% being bound at 4°C, 22.6±0.3% at 22.5°C, and 19.6±0.3% at 37°C. Derived association constants, assuming one binding site were 6.0 × 102 M−1 (4°C), 4.47 × 102 M−1 (22.5°C), and 3.88 × 102 M−1 (37°C).

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