Uric Acid: CHARACTERIZATION OF ITS INTERACTION WITH HUMAN SERUM ALBUMIN

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Abstract

Binding of sodium urate to human serum albumin (HSA) was measured by continuous ultrafiltration at pH 7.4 and ionic strength 0.16 over the concentration range 1-13 mg/100 ml. The percent sodium urate bound to 5 g/100 ml HSA was constant over this concentration range: 30.3 (SE±0.6)% being bound at 4°C, 22.6±0.3% at 22.5°C, and 19.6±0.3% at 37°C. Derived association constants, assuming one binding site were 6.0 × 102 M−1 (4°C), 4.47 × 102 M−1 (22.5°C), and 3.88 × 102 M−1 (37°C).

Authors

David S. Campion, Rodney Bluestone, James R. Klinenberg