In vivo lability of glucose-6-phosphate dehydrogenase in Gd^A- and Gd^{mediterranean} deficiency

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Abstract

A decreased level of glucose-6-phosphate dehydrogenase might result from decreased rate of synthesis, synthesis of an enzyme of lower catalytic efficiency, increased lability, or a combined mechanism. To test the hypothesis of increased lability, the rate of decline of the enzyme in vivo was measured in three groups of individuals, controls, Gd(—),A-males, and Gd(—), Mediterranean males, by the slope of decline of activity in fractions containing erythrocytes of progressively increasing mean age. These fractions were obtained by ultracentrifugation on a discontinuous density gradient of erythrocyte suspensions free of contaminating platelets and leukocytes.

Authors

Sergio Piomelli, Laurence M. Corash, Deatra D. Davenport, Janet Miraglia, Edward L. Amorosi