Gene transfer into HSCs is an effective treatment for SCID, although potentially limited by the risk of insertional mutagenesis. We performed a genome-wide analysis of retroviral vector integrations in genetically corrected HSCs and their multilineage progeny before and up to 47 months after transplantation into 5 patients with adenosine deaminase–deficient SCID. Gene-dense regions, promoters, and transcriptionally active genes were preferred retroviral integrations sites (RISs) both in preinfusion transduced CD34+ cells and in vivo after gene therapy. The occurrence of insertion sites proximal to protooncogenes or genes controlling cell growth and self renewal, including LMO2, was not associated with clonal selection or expansion in vivo. Clonal analysis of long-term repopulating cell progeny in vivo revealed highly polyclonal T cell populations and shared RISs among multiple lineages, demonstrating the engraftment of multipotent HSCs. These data have important implications for the biology of retroviral vectors, the dynamics of genetically modified HSCs, and the safety of gene therapy.
Alessandro Aiuti, Barbara Cassani, Grazia Andolfi, Massimiliano Mirolo, Luca Biasco, Alessandra Recchia, Fabrizia Urbinati, Cristina Valacca, Samantha Scaramuzza, Memet Aker, Shimon Slavin, Matteo Cazzola, Daniela Sartori, Alessandro Ambrosi, Clelia Di Serio, Maria Grazia Roncarolo, Fulvio Mavilio, Claudio Bordignon
Title and authors | Publication | Year |
---|---|---|
A Transposon and Transposase System for Human Application
PB Hackett, DA Largaespada, LJ Cooper |
Molecular Therapy | 2010 |
Dynamics of gene-modified progenitor cells analyzed by tracking retroviral integration sites in a human SCID-X1 gene therapy trial
GP Wang, CC Berry, N Malani, P Leboulch, A Fischer, S Hacein-Bey-Abina, M Cavazzana-Calvo, FD Bushman |
Blood | 2010 |
Genomic safe harbors permit high β-globin transgene expression in thalassemia induced pluripotent stem cells
EP Papapetrou, G Lee, N Malani, M Setty, I Riviere, LM Tirunagari, K Kadota, SL Roth, P Giardina, A Viale, C Leslie, FD Bushman, L Studer, M Sadelain |
Nature Biotechnology | 2010 |
Assessing the Risk of T-cell Malignancies in Mouse Models of SCID-X1
B Sorrentino |
Molecular Therapy | 2010 |
High-definition mapping of retroviral integration sites defines the fate of allogeneic T cells after donor lymphocyte infusion
C Cattoglio, G Maruggi, C Bartholomae, N Malani, D Pellin, F Cocchiarella, Z Magnani, F Ciceri, A Ambrosi, C Kalle, FD Bushman, C Bonini, M Schmidt, F Mavilio, A Recchia |
PloS one | 2010 |
Genome-wide high-throughput integrome analyses by nrLAM-PCR and next-generation sequencing
A Paruzynski, A Arens, R Gabriel, CC Bartholomae, S Scholz, W Wang, S Wolf, H Glimm, M Schmidt, C von Kalle |
Nature Protocols | 2010 |
Feline leukemia virus integrase and capsid packaging functions do not change the insertion profile of standard Moloney retroviral vectors
JY Métais, S Topp, RT Doty, B Borate, AD Nguyen, TG Wolfsberg, JL Abkowitz, CE Dunbar |
Gene Therapy | 2010 |
Gene Therapy of Chronic Granulomatous Disease: The Engraftment Dilemma
M Grez, J Reichenbach, J Schwäble, R Seger, MC Dinauer, AJ Thrasher |
Molecular Therapy | 2010 |