(A) Representative double-plotted actograms of double-housed mice that received no surgery (None, dark gray), sham surgery (light gray), or photothrombotic stroke (blue). Tan indicates lights on; white represents lights off; black tic marks denote wheel-running activity. (B) Box plot of χ² periodogram calculated rhythmicity amplitude at 24 hours. (C) Average activity counts after stroke over 24 hours. Thick lines indicate means, and shading indicates SEM. (D) Representative coronal sections of glymphatic tracer influx. Lighter colors/white are increased fluorescent tracer. Area within yellow boxes is shown at ×3 magnification to the right, with yellow arrows indicating perivascular influx of CSF tracer. (E) Box plot of MPI of glymphatic influx after sham and stroke. (F–H) Cartoon representative of the behavior tests: box plots of wire walk (F), rotarod (G), and velocity in open field (H) for sham (gray) and stroke (blue) mice. (I) Representative coronal immunohistochemistry (IHC) sections for mice 1 day, 3 days, and 11 days after photothrombotic stroke. MAP2 (magenta) was used to identify the stroke site. GFAP (yellow) and DAPI (cyan) were used for slice visualization. Scale bar: 1 mm. (J) Schematics of anterior-to-posterior coronal sections (white) with individual mouse stroke sites indicated in transparent red at 1, 3, and 11 days after stroke. The darker the red, the more stroke sites overlapped between mice. (K) Minimum/maximum box plot of stroke volume in mice 1, 3, and 11 days after stroke. In all box plots, minimum and maximum values are shown, center lines indicate the medians, quartiles are shown by box-and-whisker plots, individual mice are shown as colored dots. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. B: 1-way ANOVA test with Tukey’s HSD post hoc analysis. F–H: unpaired t tests. E: unpaired t test with Welch’s correction. K: Welch’s ANOVA test with Dunnett’s T3 multiple comparisons test. All statistics are shown in Supplemental Table 1.