(A) Module-trait correlations derived from weighted gene coexpression network analysis (WGCNA) of all expressed genes in AML cells that were either cultured alone or cocultured with CD81⁻ Erys or CD81⁺ Erys and subsequently sequenced. KEGG analysis of genes in the blue and yellow modules was performed to identify the most significantly enriched pathways. The module eigengenes (ME) represent the overall gene expression profile. (B) Identification of metabolites specifically altered in AML cells cocultured with CD81⁺ Erys. Differential abundance was determined by comparing both CD81⁺ and CD81⁻ Erys cocultures with AML monoculture. Selection criteria were set at a raw P value less than 0.05 and an FDR less than 0.2 for both comparisons. (C) Classification of differentially expressed metabolites identified in AML cells cocultured with CD81⁺ Erys using the ClassyFire superclass annotation. (D) Protein expression levels of LPCAT1, LPCAT3, and LPCAT4 in AML cells cultured alone or cocultured with CD81⁻ Erys or CD81⁺ Erys. β-Actin was used as a loading control. Molecular weights are indicated on the right. (E) A schematic illustrating the experimental design for lysophosphatidylcholine (LysoPC) supplementation in AML monoculture. (F) Cell counts of AML cells obtained after 6 days of culture (n = 4). (G) Number of colonies formed by cultured AML cells (n = 4). (H) A schematic illustrating the experimental design: AML cells were cocultured with CD81⁻ Erys or CD81⁺ Erys, or cultured alone, for 6 days, with or without LysoPC supplementation. (I) Cell counts of AML cells obtained after 6 days of culture (n = 5). Data are presented as the mean ± SEM. The results shown are representative of 1 of 3 independent experiments with consistent trends. ***P < 0.001, by 2-tailed, unpaired t test (F and G) and 1-way ANOVA (I).