(A) Schematic representation of full-length and truncated forms of rat gravin-α. The 10–amino acid HDAC11 binding domain is indicated. (B) Coimmunoprecipitation of Myc-tagged HDAC11 with full-length (WT) and truncated forms of gravin-α in transfected HEK293 cells; gravin-α migrates as a monomer (M) and oligomer (O) in SDS-PAGE. (C) Schematic depiction of Gravi-D. (D) Schematic depiction of the lipid raft protein coimmunoprecipitation study. (E) Coimmunoprecipitation analysis of the indicated proteins. (F) 3T3-L1 adipocytes were treated with vehicle control or CL-316,243 (1 μM) for 30 minutes followed by scrambled peptide or Gravi-D for 1 additional hour, and harvested for immunoblotting. (G) Immunofluorescence images of 3T3-L1 adipocytes treated with vehicle (water), scrambled peptide (1 μM), or Gravi-D (1 μM) for 1 hour. Scale bars: 50 μm. (H) A model for Gravi-D–mediated induction of thermogenesis and lipolysis. For all blots, each lane represents protein from an independent plate of cells.