Rates of ROM release by neutrophils from nonpregnant (a and c) and pregnant subjects (b and d). ROM release was measured during cell migration through a matrix containing H2TMRos (35). For all panels, the cells are adherent and traces labeled 1–9 are: 1, untreated; 2, LPS; 3, IFN-γ; 4, 6-AN; 5, IFN-γ plus LPS; 6, LPS plus 6-AN; 7, colchicine; 8, LPS plus colchicine; and 9, LPS plus colchicine and 6-AN. The greatest rates of ROM release from pregnancy neutrophils require the addition of colchicine. See Table 1 for further quantitative results. Vertical bar = 10,000 counts.