Molecular mechanisms of blister formation in bullous impetigo and staphylococcal scalded skin syndrome
Yasushi Hanakawa, Norman M. Schechter, Chenyan Lin, Luis Garza, Hong Li, Takayuki Yamaguchi, Yasuyuki Fudaba, Koji Nishifuji, Motoyuki Sugai, Masayuki Amagai, John R. Stanley
Yasushi Hanakawa, Norman M. Schechter, Chenyan Lin, Luis Garza, Hong Li, Takayuki Yamaguchi, Yasuyuki Fudaba, Koji Nishifuji, Motoyuki Sugai, Masayuki Amagai, John R. Stanley
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Article Infectious disease

Molecular mechanisms of blister formation in bullous impetigo and staphylococcal scalded skin syndrome

Abstract

Research Article

Authors

Yasushi Hanakawa, Norman M. Schechter, Chenyan Lin, Luis Garza, Hong Li, Takayuki Yamaguchi, Yasuyuki Fudaba, Koji Nishifuji, Motoyuki Sugai, Masayuki Amagai, John R. Stanley

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Coprecipitation of ETs with Dsg1. (a) Immunoprecipitation followed by im...
Coprecipitation of ETs with Dsg1. (a) Immunoprecipitation followed by immunoblotting. Incubation of ETB (marked with a FLAG epitope tag) and ETD with Dsg1 showed coprecipitation at 1 minute that was markedly diminished at 60 minutes, after increased cleavage of Dsg1. Similar results were found for ETA (data not shown). (b) Binding of Dsg1 with ETA Cmu and ETA Amu. ETA Cmu, which slowly cleaves Dsg1, showed decreased binding with time. (c) ETA and ETB (marked with His epitope tags) bind to Dsg1 but not Dsg3. These ETs did not cleave or bind to Dsg3, which is highly homologous in amino acid sequence to Dsg1. Similar results were found with ETD (data not shown). IP, immunoprecipitation; IB, immunoblot.