Adenosine kinase inhibition promotes survival of fetal adenosine deaminase–deficient thymocytes by blocking dATP accumulation
C. Justin Van De Wiele, … , Hong Jiang, Linda F. Thompson
C. Justin Van De Wiele, … , Hong Jiang, Linda F. Thompson
Published August 1, 2002
Citation Information: J Clin Invest. 2002;110(3):395-402. https://doi.org/10.1172/JCI15683.
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Article Genetics

Adenosine kinase inhibition promotes survival of fetal adenosine deaminase–deficient thymocytes by blocking dATP accumulation

Abstract

Research Article

Authors

C. Justin Van De Wiele, James G. Vaughn, Michael R. Blackburn, Catherine A. Ledent, Marlene Jacobson, Hong Jiang, Linda F. Thompson

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Inhibition of adenosine kinase rescues thymocyte development within ADA-...
Inhibition of adenosine kinase rescues thymocyte development within ADA-inhibited FTOC. FTOCs were performed with C57BL/6 thymuses at day 15 of gestation in media alone (a), 5 μM dCF (b), 1 μM 5′A5′dAdo (c), or 5 μM dCF plus 1 μM 5′A5′dAdo (d). After 5 days, thymocytes were harvested, counted, and stained with FITC-anti-CD4, PE-anti-CD8, and PI to exclude dead cells. The data are representative of one experiment out of four.