CLA and CLA-ligand expression in skin and by lesion-derived cells. (a) Frozen section of HSV-2 lesion from subject 5491 stained with anti–E-selectin and hematoxylin. Epidermis oriented to the top and original magnification ×200 for all panels. (b) Normal skin from subject 5491 stained with anti–E-selectin. (c) HSV-2 lesion stained with anti-CLA. (d) Normal skin stained with anti-CLA. (e) HSV-2 lesion stained with hematoxylin and eosin. (f) Normal skin stained with hematoxylin and eosin. (g) Lymphocytes expanded for 11 days from the biopsy stained with HSV-2–specific tetramers B7-RPR-PE (left) or B7-APA-PE (right) and anti-CD8α. Quadrant lines are cutoffs for CD8α-high cells and tetramer binding. The percentages of CD8α-high cells that are tetramer-positive are indicated. (h) Expression of CLA by CD8α-high, tetramer-binding cells for tetramer B7-RPR-PE (left) or B7-APA-PE (right). (i) Expression of CLA by CD8α-high, tetramer nonbinding cells. Percentages of CLA-positive cells are indicated.