Shouhong Xuan, Tadahiro Kitamura, Jun Nakae, Katerina Politi, Yoshiaki Kido, Peter E. Fisher, Manrico Morroni, Saverio Cinti, Morris F. White, Pedro L. Herrera, Domenico Accili, Argiris Efstratiadis
Ki67, histone H3, and BrdU labeling of β cells. Adjacent 4-μm-thick pancreatic section from mice at E18.5, P10, P30, and P120 were costained with anti-insulin Ab’s (to identify β cells) and anti-Ki67, or anti-histone H3 or anti-BrdU Ab’s (to provide an index of proliferation). Positive cells were visually scored, and the number of Ki67-positive cells was plotted as a function of the number of histone H3–positive (upper panel) or BrdU-positive cells (lower panel). Regression analysis was performed using the Statsview software. r2 = 0.9 for the H3/Ki67 and 0.9 for the BrdU/Ki67 correlation (P < 0.0001).