Establishment of the human placenta requires that fetal cytotrophoblast stem cells in anchoring chorionic villi become invasive. These cytotrophoblasts aggregate into cell columns and invade both the uterine interstitium and vasculature, anchoring the fetus to the mother and establishing blood flow to the placenta. Cytotrophoblasts colonizing spiral arterioles replace maternal endothelium as far as the first third of the myometrium. We show here that differentiating cytotrophoblasts transform their adhesion receptor phenotype so as to resemble the endothelial cells they replace. Cytotrophoblasts in cell columns show reduced E-cadherin staining and express VE-(endothelial) cadherin, platelet-endothelial adhesion molecule-1, vascular endothelial adhesion molecule-1, and alpha-4-integrins. Cytotrophoblasts in the uterine interstitium and maternal vasculature continue to express these receptors, and, like endothelial cells during angiogenesis, also stain for alphaVbeta3. In functional studies, alphaVbeta3 and VE-cadherin enhance, while E-cadherin restrains, cytotrophoblast invasiveness. Cytotrophoblasts expressing alpha4 integrins bound immobilized VCAM-1 in vitro, suggesting that this receptor-pair could mediate cytotrophoblast-endothelium or cytotrophoblast-cytotrophoblast interactions in vivo, during endovascular invasion. In the pregnancy disorder preeclampsia, in which endovascular invasion remains superficial, cytotrophoblasts fail to express most of these endothelial markers (Zhou et al., 1997. J. Clin. Invest. 99:2152-2164.), suggesting that this adhesion phenotype switch is required for successful endovascular invasion and normal placentation.
Y Zhou, S J Fisher, M Janatpour, O Genbacev, E Dejana, M Wheelock, C H Damsky
Title and authors | Publication | Year |
---|---|---|
Methods in Enzymology
B Shao, JW Heinecke |
Methods in enzymology | 2008 |
Deficiency in catechol-O-methyltransferase and 2-methoxyoestradiol is associated with pre-eclampsia
RA Irani, Y Zhang, SC Blackwell, CC Zhou, SM Ramin, RE Kellems, Y Xia |
Nature | 2008 |
Deficiency in catechol-O-methyltransferase and 2-methoxyoestradiol is associated with pre-eclampsia
RA Irani, Y Zhang, SC Blackwell, CC Zhou, SM Ramin, RE Kellems, Y Xia |
Nature | 2008 |
Molecular mechanisms of preeclampsia
WP Mutter, SA Karumanchi |
Microvascular Research | 2008 |
Autoantibody From Women With Preeclampsia Induces Soluble Fms-Like Tyrosine Kinase-1 Production via Angiotensin Type 1 Receptor and Calcineurin/Nuclear Factor of Activated T-Cells Signaling
CC Zhou, S Ahmad, T Mi, S Abbasi, L Xia, MC Day, SM Ramin, A Ahmed, RE Kellems, Y Xia |
Hypertension | 2008 |
Series in Maternal-Fetal Medicine
BE Metzger |
Textbook of Diabetes and Pregnancy | 2008 |
Reproductive Medicine and Assisted Reproductive Techniques
FL Lopes, J Desmarais, BD Murphy |
Endometrium, The | 2008 |
Ultrastructural localization of glycoprotein IIIa (GPIIIa, β3 integrin) on placental syncytiotrophoblast microvilli: implications for platelet alloimmunization during pregnancy
BM Kumpel, K Sibley, DJ Jackson, G White, PW Soothill |
Transfusion | 2008 |
ORIGINAL ARTICLE: Trophoblasts and Shear Stress Induce an Asymmetric Distribution of ICAM-1 in Uterine Endothelial Cells
TC Cao, TL Thirkill, M Wells, AI Barakat, GC Douglas |
American Journal of Reproductive Immunology | 2008 |
Blockade of adhesion molecule CD146 causes pregnancy failure in mice
Q Liu, B Zhang, X Zhao, Y Zhang, Y Liu, X Yan |
Journal of Cellular Physiology | 2008 |
Decidual endothelial cells express surface-bound C1q as a molecular bridge between endovascular trophoblast and decidual endothelium
R Bulla, C Agostinis, F Bossi, L Rizzi, A Debeus, C Tripodo, O Radillo, FD Seta, B Ghebrehiwet, F Tedesco |
Molecular Immunology | 2008 |
Systemic and uteroplacental renin--angiotensin system in normal and pre-eclamptic pregnancies
L Anton, KB Brosnihan |
Therapeutic advances in cardiovascular disease | 2008 |
Endothelial adherens and tight junctions in vascular homeostasis, inflammation and angiogenesis
Y Wallez, P Huber |
Biochimica et Biophysica Acta (BBA) - Biomembranes | 2008 |
Genetic and epigenetic factors contribute to the onset of preeclampsia
ST Chelbi, D Vaiman |
Molecular and Cellular Endocrinology | 2008 |
In vitro spheroid model of placental vasculogenesis: does it work?
N Baal, R Widmer-Teske, T McKinnon, KT Preissner, MT Zygmunt |
Laboratory Investigation | 2008 |
Preeclampsia and Angiogenic Imbalance
S Maynard, FH Epstein, SA Karumanchi |
Annual Review of Medicine | 2008 |
Targeting Cancer Stem Cells to Modulate Alternative Vascularization Mechanisms
E Monzani, CA Porta |
Stem Cell Reviews | 2008 |
Pathophysiology of placentation abnormalities in pregnancy-induced hypertension
M Furuya, J Ishida, I Aoki, A Fukamizu |
Vascular Health and Risk Management | 2008 |
An alternatively spliced isoform of PECAM-1 is expressed at high levels in human and murine tissues, and suggests a novel role for the C-terminus of PECAM-1 in cytoprotective signaling
C Bergom, C Paddock, C Gao, T Holyst, DK Newman, PJ Newman |
Journal of cell science | 2008 |
Correlation between factors involved in the local haemostasis and angiogenesis in full term human placenta
E Chinni, D Colaizzo, M Margaglione, C Rubini, RL D'Ambrosio, F Giuliani, GD Vagno, E Grandone |
Thrombosis Research | 2008 |
Uric Acid as a Pathogenic Factor in Preeclampsia
SA Bainbridge, JM Roberts |
Placenta | 2008 |
Reproductive Medicine and Assisted Reproductive Techniques: Molecular, Cellular, and Clinical Perspectives
VD Winn, K Red-Horse, SJ Fisher |
Reproductive Medicine and Assisted Reproductive Techniques: Molecular, Cellular, and Clinical Perspectives | 2008 |
Hypertensions de la grossesse
M Beaufils |
EMC - Cardiologie | 2008 |
In Vitro and In Vivo Evidence for Lack of Endovascular Remodeling by Third Trimester Trophoblasts
S Kalkunte, Z Lai, N Tewari, C Chichester, R Romero, J Padbury, S Sharma |
Placenta | 2008 |
Placenta Accreta and Cesarean Scar Pregnancy: Overlooked Costs of the Rising Cesarean Section Rate
T Rosen |
Clinics in Perinatology | 2008 |
Endovascular Trophoblast Invasion, Spiral Artery Remodelling and Uteroplacental Haemodynamics in a Transgenic Rat Model of Pre-eclampsia
N Geusens, S Verlohren, C Luyten, M Taube, L Hering, L Vercruysse, M Hanssens, JW Dudenhausen, R Dechend, R Pijnenborg |
Placenta | 2008 |
Accumulation of Uterine CD16(−) Natural Killer (NK) Cells: Friends, Foes, or Jekyll-and-Hyde Relationship for the Conceptus?
K Kitaya |
Immunological Investigations | 2008 |
Preeclampsia and small-for-gestational age are associated with decreased concentrations of a factor involved in angiogenesis: Soluble Tie-2
F Gotsch, R Romero, JP Kusanovic, T Chaiworapongsa, M Dombrowski, O Erez, NG Than, S Mazaki-Tovi, P Mittal, J Espinoza, SS Hassan |
Journal of Maternal-Fetal and Neonatal Medicine | 2008 |
The Role of Decidual Natural Killer Cells in Normal Placentation and in the Pathogenesis of Preeclampsia
G Eastabrook, Y Hu, P Dadelszen |
Journal of Obstetrics and Gynaecology Canada | 2008 |
Review: Systemic and uteroplacental renin—angiotensin system in normal and pre-eclamptic pregnancies
L Anton, KB Brosnihan |
Therapeutic advances in cardiovascular disease | 2008 |
Cytokine Regulation of Intercellular Adhesion Molecule-1 Expression on Trophoblasts in Preeclampsia
E Abe, K Matsubara, K Oka, Y Kusanagi, M Ito |
Gynecologic and Obstetric Investigation | 2008 |
Decorin-Mediated Inhibition of Proliferation and Migration of the Human Trophoblast via Different Tyrosine Kinase Receptors
D Iacob, J Cai, M Tsonis, A Babwah, C Chakraborty, RN Bhattacharjee, PK Lala |
Endocrinology | 2008 |
Role of EG-VEGF in human placentation: Physiological and pathological implications
P Hoffmann, Y Saoudi, M Benharouga, CH Graham, JP Schaal, C Mazouni, JJ Feige, N Alfaidy |
Journal of Cellular and Molecular Medicine | 2008 |
Human Cytomegalovirus
TE Shenk, MF Stinski |
2008 | |
Targeting Cancer Stem Cells to Modulate Alternative Vascularization Mechanisms
E Monzani, CA Porta |
Stem Cell Reviews and Reports | 2008 |