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Citations to this article

TGF-beta 1 and 25-hydroxycholesterol stimulate osteoblast-like vascular cells to calcify.
K E Watson, … , B Norton, L L Demer
K E Watson, … , B Norton, L L Demer
Published May 1, 1994
Citation Information: J Clin Invest. 1994;93(5):2106-2113. https://doi.org/10.1172/JCI117205.
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Research Article Article has an altmetric score of 6

TGF-beta 1 and 25-hydroxycholesterol stimulate osteoblast-like vascular cells to calcify.

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Abstract

Previous studies in our laboratory demonstrated messenger RNA for bone morphogenetic protein-2a in human calcified plaque, suggesting that arterial calcification is a regulated process, similar to osteogenesis. To further test this hypothesis, we have isolated and cloned a subpopulation of cells from bovine aortic media that show osteoblastic potential. These novel cells are primarily distinguished from smooth muscle cells by expression of a surface marker preliminarily identified as a modified form of the ganglioside sialyl-lactosylceramide (GM3). Osteoblastic potential was indicated by high levels of alkaline phosphatase and collagen I, expression of osteopontin and osteonectin (SPARC), and production of bone-specific osteocalcin and hydroxyapatite. Cultures of these cells were stimulated to form increased numbers of calcium-mineral-producing nodules by the oxysterol 25-hydroxycholesterol as well as by transforming growth factor-beta 1, both known to be present in atherosclerotic lesions. The stimulation of calcifying vascular cells in the artery wall by these two factors suggests a possible mechanism for the colocalization of calcification with atherosclerosis in vivo.

Authors

K E Watson, K Boström, R Ravindranath, T Lam, B Norton, L L Demer

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Citations to this article in year 2020 (9)

Title and authors Publication Year
Stochastic non-enzymatic modification of long-lived macromolecules - A missing hallmark of aging
A Fedintsev, A Moskalev
Ageing Research Reviews 2020
The role of bone morphogenetic protein signaling in vascular calcification
P Yang, L Troncone, ZM Augur, SS Kim, ME McNeil, PB Yu
Bone 2020
E3 Ubiquitin ligase NEDD4 family‑regulatory network in cardiovascular disease
Y Zhang, H Qian, B Wu, S You, S Wu, S Lu, P Wang, L Cao, N Zhang, Y Sun
International journal of biological sciences 2020
Cardiovascular Calcification and Bone Mineralization
E Aikawa, JD Hutcheson
2020
Multiple Pathways for Pathological Calcification in the Human Body
N Vidavsky, JA Kunitake, LA Estroff
Advanced Healthcare Materials 2020
25-Hydroxycholesterol promotes vascular calcification via activation of endoplasmic reticulum stress
Q Dong, Y Chen, W Liu, X Liu, A Chen, X Yang, Y Li, S Wang, M Fu, JS Ou, L Lu, J Yan
European Journal of Pharmacology 2020
An Investigation Into the Role of Osteocalcin in Human Arterial Smooth Muscle Cell Calcification
SA Millar, SG John, CW McIntyre, V Ralevic, SI Anderson, SE O'Sullivan
Frontiers in Endocrinology 2020
Efficacy of Collagenase Clostridium histolyticum (Xiapex®) in Patients with the Acute Phase of Peyronie’s Disease
A Cocci, FD Maida, GI Russo, P Capogrosso, L Francesco, M Rizzo, MD Mauro, A Salonia, G Cito, M Falcone, A Romano, G Polloni, JI Martinez-Salamanca, E Fernández-Pascual, A Minervini, N Mondaini
Clinical Drug Investigation 2020
Kalp Kapakları Bize ne Diyor?
S KOÇARSLAN, A KOÇARSLAN, M KİRİŞÇİ, E EROĞLU, M ACİPAYAM, A YASIM
2020

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Referenced in 29 patents
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