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Citations to this article

TGF-beta 1 and 25-hydroxycholesterol stimulate osteoblast-like vascular cells to calcify.
K E Watson, … , B Norton, L L Demer
K E Watson, … , B Norton, L L Demer
Published May 1, 1994
Citation Information: J Clin Invest. 1994;93(5):2106-2113. https://doi.org/10.1172/JCI117205.
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Research Article Article has an altmetric score of 6

TGF-beta 1 and 25-hydroxycholesterol stimulate osteoblast-like vascular cells to calcify.

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Abstract

Previous studies in our laboratory demonstrated messenger RNA for bone morphogenetic protein-2a in human calcified plaque, suggesting that arterial calcification is a regulated process, similar to osteogenesis. To further test this hypothesis, we have isolated and cloned a subpopulation of cells from bovine aortic media that show osteoblastic potential. These novel cells are primarily distinguished from smooth muscle cells by expression of a surface marker preliminarily identified as a modified form of the ganglioside sialyl-lactosylceramide (GM3). Osteoblastic potential was indicated by high levels of alkaline phosphatase and collagen I, expression of osteopontin and osteonectin (SPARC), and production of bone-specific osteocalcin and hydroxyapatite. Cultures of these cells were stimulated to form increased numbers of calcium-mineral-producing nodules by the oxysterol 25-hydroxycholesterol as well as by transforming growth factor-beta 1, both known to be present in atherosclerotic lesions. The stimulation of calcifying vascular cells in the artery wall by these two factors suggests a possible mechanism for the colocalization of calcification with atherosclerosis in vivo.

Authors

K E Watson, K Boström, R Ravindranath, T Lam, B Norton, L L Demer

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Citations to this article in year 2004 (18)

Title and authors Publication Year
Vascular calcification in chronic kidney disease
WG Goodman, G London, K Amann, GA Block, C Giachelli, KA Hruska, M Ketteler, A Levin, Z Massy, DA McCarron, P Raggi, CM Shanahan, N Yorioka
American Journal of Kidney Diseases 2004
When man turns to stone: Extraosseous calcification in uremic patients
J Floege
Kidney International 2004
Cardiovascular calcification in patients with chronic renal failure: Are we on target with this risk factor?
PA McCullough, S Soman
Kidney International 2004
Inhibitory Effects of Etidronate on the Progression of Vascular Calcification in Hemodialysis Patients
H Hashiba, S Aizawa, K Tamura, T Shigematsu, H Kogo
Therapeutic Apheresis and Dialysis 2004
Aortic Calcification Is Associated With Age and Sex but Not Left Ventricular Mass in Essential Hypertension
A Tsakiris, M Doumas, N Nearchos, A Mavrokefalos, N Mpatakis, P Skoufas
The Journal of Clinical Hypertension 2004
Association between cardiac valvular calcification and coronary artery disease in a low-risk population
HK Kim, SJ Park, JW Suh, YJ Kim, HS Kim, DW Sohn, BH Oh, MM Lee, YB Park, YS Choi
Coronary Artery Disease 2004
ChIP Display: novel method for identification of genomic targets of transcription factors
A Barski, B Frenkel
Nucleic Acids Research 2004
Mechanical stimulation and mitogen-activated protein kinase signaling independently regulate osteogenic differentiation and mineralization by calcifying vascular cells
CA Simmons, J Nikolovski, AJ Thornton, S Matlis, DJ Mooney
Journal of Biomechanics 2004
Mechanisms of aortic valve calcification
ER Mohler
The American Journal of Cardiology 2004
Vascular smooth muscle cells and calcification in atherosclerosis
A Trion, A der Laarse
American Heart Journal 2004
Role of inflammation in atherosclerotic calcification, metaplasia and osteoporosis
LL Demer, Y Tintut, M Abedin
International Congress Series 2004
Regulation of cardiovascular calcification
MY Speer, CM Giachelli
Cardiovascular Pathology 2004
Cholestane-3β, 5α, 6β-triol promotes vascular smooth muscle cells calcification
H Liu, L Yuan, S Xu, T Zhang, K Wang
Life Sciences 2004
Mechanism of dystrophic calcification in rabbit aortas
HH Hsu, O Tawfik, F Sun
Cardiovascular Pathology 2004
Role of bicarbonate/CO2 buffer in the initiation of vesicle-mediated calcification: mechanisms of aortic calcification related to atherosclerosis
HH Hsu, BG Abbo
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 2004
Coronary Radiology
M Oudkerk
2004
Osteoporosis and Cardiovascular Disease: Brittle Bones and Boned Arteries, Is There a Link?
SI McFarlane, R Muniyappa, JJ Shin, G Bahtiyar, JR Sowers
Endocrine 2004
Aortic calcification is associated with age and sex but not left ventricular mass in essential hypertension.
Tsakiris A, Doumas M, Nearchos N, Mavrokefalos A, Mpatakis N, Skoufas P
Journal of clinical hypertension (Greenwich, Conn.) 2004

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