Previous studies in our laboratory demonstrated messenger RNA for bone morphogenetic protein-2a in human calcified plaque, suggesting that arterial calcification is a regulated process, similar to osteogenesis. To further test this hypothesis, we have isolated and cloned a subpopulation of cells from bovine aortic media that show osteoblastic potential. These novel cells are primarily distinguished from smooth muscle cells by expression of a surface marker preliminarily identified as a modified form of the ganglioside sialyl-lactosylceramide (GM3). Osteoblastic potential was indicated by high levels of alkaline phosphatase and collagen I, expression of osteopontin and osteonectin (SPARC), and production of bone-specific osteocalcin and hydroxyapatite. Cultures of these cells were stimulated to form increased numbers of calcium-mineral-producing nodules by the oxysterol 25-hydroxycholesterol as well as by transforming growth factor-beta 1, both known to be present in atherosclerotic lesions. The stimulation of calcifying vascular cells in the artery wall by these two factors suggests a possible mechanism for the colocalization of calcification with atherosclerosis in vivo.
K E Watson, K Boström, R Ravindranath, T Lam, B Norton, L L Demer
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Cardiovascular calcification in patients with chronic renal failure: Are we on target with this risk factor?
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Kidney International | 2004 |
Inhibitory Effects of Etidronate on the Progression of Vascular Calcification in Hemodialysis Patients
H Hashiba, S Aizawa, K Tamura, T Shigematsu, H Kogo |
Therapeutic Apheresis and Dialysis | 2004 |
Aortic Calcification Is Associated With Age and Sex but Not Left Ventricular Mass in Essential Hypertension
A Tsakiris, M Doumas, N Nearchos, A Mavrokefalos, N Mpatakis, P Skoufas |
The Journal of Clinical Hypertension | 2004 |
Association between cardiac valvular calcification and coronary artery disease in a low-risk population
HK Kim, SJ Park, JW Suh, YJ Kim, HS Kim, DW Sohn, BH Oh, MM Lee, YB Park, YS Choi |
Coronary Artery Disease | 2004 |
ChIP Display: novel method for identification of genomic targets of transcription factors
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Nucleic Acids Research | 2004 |
Mechanical stimulation and mitogen-activated protein kinase signaling independently regulate osteogenic differentiation and mineralization by calcifying vascular cells
CA Simmons, J Nikolovski, AJ Thornton, S Matlis, DJ Mooney |
Journal of Biomechanics | 2004 |
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ER Mohler |
The American Journal of Cardiology | 2004 |
Vascular smooth muscle cells and calcification in atherosclerosis
A Trion, A der Laarse |
American Heart Journal | 2004 |
Role of inflammation in atherosclerotic calcification, metaplasia and osteoporosis
LL Demer, Y Tintut, M Abedin |
International Congress Series | 2004 |
Regulation of cardiovascular calcification
MY Speer, CM Giachelli |
Cardiovascular Pathology | 2004 |
Cholestane-3β, 5α, 6β-triol promotes vascular smooth muscle cells calcification
H Liu, L Yuan, S Xu, T Zhang, K Wang |
Life Sciences | 2004 |
Mechanism of dystrophic calcification in rabbit aortas
HH Hsu, O Tawfik, F Sun |
Cardiovascular Pathology | 2004 |
Role of bicarbonate/CO2 buffer in the initiation of vesicle-mediated calcification: mechanisms of aortic calcification related to atherosclerosis
HH Hsu, BG Abbo |
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease | 2004 |
Coronary Radiology
M Oudkerk |
2004 | |
Osteoporosis and Cardiovascular Disease: Brittle Bones and Boned Arteries, Is There a Link?
SI McFarlane, R Muniyappa, JJ Shin, G Bahtiyar, JR Sowers |
Endocrine | 2004 |
Aortic calcification is associated with age and sex but not left ventricular mass in essential hypertension.
Tsakiris A, Doumas M, Nearchos N, Mavrokefalos A, Mpatakis N, Skoufas P |
Journal of clinical hypertension (Greenwich, Conn.) | 2004 |