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Citations to this article

Phenotypes of apolipoprotein B and apolipoprotein E after liver transplantation.
M F Linton, … , M R Wardell, S G Young
M F Linton, … , M R Wardell, S G Young
Published July 1, 1991
Citation Information: J Clin Invest. 1991;88(1):270-281. https://doi.org/10.1172/JCI115288.
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Research Article Article has an altmetric score of 6

Phenotypes of apolipoprotein B and apolipoprotein E after liver transplantation.

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Abstract

Apolipoprotein (apo) E and the two B apolipoproteins, apoB48 and apoB100, are important proteins in human lipoprotein metabolism. Commonly occurring polymorphisms in the genes for apoE and apoB result in amino acid substitutions that produce readily detectable phenotypic differences in these proteins. We studied changes in apoE and apoB phenotypes before and after liver transplantation to gain new insights into apolipoprotein physiology. In all 29 patients that we studied, the postoperative serum apoE phenotype of the recipient, as assessed by isoelectric focusing, converted virtually completely to that of the donor, providing evidence that greater than 90% of the apoE in the plasma is synthesized by the liver. In contrast, the cerebrospinal fluid apoE phenotype did not change to the donor's phenotype after liver transplantation, indicating that most of the apoE in CSF cannot be derived from the plasma pool and therefore must be synthesized locally. The apoB100 phenotype (assessed with immunoassays using monoclonal antibody MB19, an antibody that detects a two-allele polymorphism in apoB) invariably converted to the phenotype of the donor. In four normolipidemic patients, we determined the MB19 phenotype of both the apoB100 and apoB48 in the "chylomicron fraction" isolated from plasma 3 h after a fat-rich meal. Interestingly, the apoB100 in the chylomicron fraction invariably had the phenotype of the donor, indicating that the vast majority of the large, triglyceride-rich apoB100-containing lipoproteins that appear in the plasma after a fat-rich meal are actually VLDL of hepatic origin. The MB19 phenotype of the apoB48 in the plasma chylomicron fraction did not change after liver transplantation, indicating that almost all of the apoB48 in plasma chylomicrons is derived from the intestine. These results were consistent with our immunocytochemical studies on intestinal biopsy specimens of organ donors; using apoB-specific monoclonal antibodies, we found evidence for apoB48, but not apoB100, in donor intestinal biopsy specimens.

Authors

M F Linton, R Gish, S T Hubl, E Bütler, C Esquivel, W I Bry, J K Boyles, M R Wardell, S G Young

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Citations to this article in year 2020 (16)

Title and authors Publication Year
ApoE mimetic improves pathology and memory in a model of Alzheimer’s disease
K Krishnamurthy, V Cantillana, H Wang, PM Sullivan, BJ Kolls, X Ge, Y Lin, B Mace, DT Laskowitz
Brain Research 2020
Apolipoprotein E Signals via TLR4 to Induce CXCL5 Secretion by Asthmatic Airway Epithelial Cells
O Kalchiem-Dekel, X Yao, AV Barochia, M Kaler, DM Figueroa, WB Karkowsky, EM Gordon, M Gao, MM Fergusson, X Qu, P Liu, Y Li, F Seifuddin, M Pirooznia, SJ Levine
American journal of respiratory cell and molecular biology 2020
APOE2: protective mechanism and therapeutic implications for Alzheimer’s disease
Z Li, F Shue, N Zhao, M Shinohara, G Bu
Molecular Neurodegeneration 2020
Therapeutic approaches targeting Apolipoprotein E function in Alzheimer’s disease
T Williams, DR Borchelt, P Chakrabarty
Molecular Neurodegeneration 2020
Transendothelial transport of lipoproteins
E Jang, J Robert, L Rohrer, A von Eckardstein, WL Lee
Atherosclerosis 2020
Impact of apolipoprotein E genetic polymorphisms on liver disease: An essential review
JC Nascimento, GA Matos, LC Pereira, AE Mourão, AM Sampaio, RB Oriá, P Toniutto
ANN HEPATOL 2020
The Role of HDL and HDL Mimetic Peptides as Potential Therapeutics for Alzheimer’s Disease
D Chernick, R Zhong, L Li
Biomolecules 2020
Association of Apolipoprotein E in Lipoprotein Subspecies With Risk of Dementia
M Koch, ST DeKosky, M Goodman, J Sun, JD Furtado, AL Fitzpatrick, RH Mackey, T Cai, OL Lopez, LH Kuller, KJ Mukamal, MK Jensen
2020
Sphingolipids in Alzheimer's disease, how can we target them?
SM Crivelli, C Giovagnoni, L Visseren, AL Scheithauer, N de Wit, S den Hoedt, M Losen, MT Mulder, J Walter, HE de Vries, E Bieberich, P Martinez-Martinez
Advanced Drug Delivery Reviews 2020
Cerebrovascular amyloid Angiopathy in bioengineered vessels is reduced by high-density lipoprotein particles enriched in Apolipoprotein E
J Robert, EB Button, EM Martin, L McAlary, Z Gidden, M Gilmour, G Boyce, TM Caffrey, A Agbay, A Clark, JM Silverman, NR Cashman, CL Wellington
Molecular Neurodegeneration 2020
Pulsed SILAM Reveals In Vivo Dynamics of Murine Brain Protein Translation
SS Ng, JE Park, W Meng, CP Chen, RN Kalaria, NE McCarthy, SK Sze
ACS Omega 2020
Beyond the CNS: The many peripheral roles of APOE
AB Martinez-Martínez, E Torres-Perez, N Devanney, RD Moral, LA Johnson, JM Arbones-Mainar
Neurobiology of Disease 2020
Therapeutic Development of Apolipoprotein E Mimetics for Acute Brain Injury: Augmenting Endogenous Responses to Reduce Secondary Injury
ML James, JM Komisarow, H Wang, DT Laskowitz
Neurotherapeutics 2020
An in vitro bioengineered model of the human arterial neurovascular unit to study neurodegenerative diseases
J Robert, NL Weilinger, LP Cao, S Cataldi, EB Button, S Stukas, EM Martin, P Seibler, M Gilmour, TM Caffrey, EM Rowe, J Fan, B MacVicar, MJ Farrer, CL Wellington
Molecular Neurodegeneration 2020
Validation of a novel and accurate ApoE4 assay for automated chemistry analyzers
S Veiga, A Rodríguez-Martín, G Garcia-Ribas, I Arribas, M Menacho-Román, M Calero
Scientific Reports 2020
High‐density lipoprotein‐related cholesterol metabolism in Alzheimer’s disease
S Pedrini, P Chatterjee, E Hone, RN Martins
Journal of Neurochemistry 2020

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