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Citations to this article

Phenotypes of apolipoprotein B and apolipoprotein E after liver transplantation.
M F Linton, … , M R Wardell, S G Young
M F Linton, … , M R Wardell, S G Young
Published July 1, 1991
Citation Information: J Clin Invest. 1991;88(1):270-281. https://doi.org/10.1172/JCI115288.
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Research Article Article has an altmetric score of 6

Phenotypes of apolipoprotein B and apolipoprotein E after liver transplantation.

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Abstract

Apolipoprotein (apo) E and the two B apolipoproteins, apoB48 and apoB100, are important proteins in human lipoprotein metabolism. Commonly occurring polymorphisms in the genes for apoE and apoB result in amino acid substitutions that produce readily detectable phenotypic differences in these proteins. We studied changes in apoE and apoB phenotypes before and after liver transplantation to gain new insights into apolipoprotein physiology. In all 29 patients that we studied, the postoperative serum apoE phenotype of the recipient, as assessed by isoelectric focusing, converted virtually completely to that of the donor, providing evidence that greater than 90% of the apoE in the plasma is synthesized by the liver. In contrast, the cerebrospinal fluid apoE phenotype did not change to the donor's phenotype after liver transplantation, indicating that most of the apoE in CSF cannot be derived from the plasma pool and therefore must be synthesized locally. The apoB100 phenotype (assessed with immunoassays using monoclonal antibody MB19, an antibody that detects a two-allele polymorphism in apoB) invariably converted to the phenotype of the donor. In four normolipidemic patients, we determined the MB19 phenotype of both the apoB100 and apoB48 in the "chylomicron fraction" isolated from plasma 3 h after a fat-rich meal. Interestingly, the apoB100 in the chylomicron fraction invariably had the phenotype of the donor, indicating that the vast majority of the large, triglyceride-rich apoB100-containing lipoproteins that appear in the plasma after a fat-rich meal are actually VLDL of hepatic origin. The MB19 phenotype of the apoB48 in the plasma chylomicron fraction did not change after liver transplantation, indicating that almost all of the apoB48 in plasma chylomicrons is derived from the intestine. These results were consistent with our immunocytochemical studies on intestinal biopsy specimens of organ donors; using apoB-specific monoclonal antibodies, we found evidence for apoB48, but not apoB100, in donor intestinal biopsy specimens.

Authors

M F Linton, R Gish, S T Hubl, E Bütler, C Esquivel, W I Bry, J K Boyles, M R Wardell, S G Young

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 2000 1999 1998 1997 1996 1995 1994 1993 1992 1986 Total
Citations: 3 9 14 17 11 16 9 5 7 9 10 12 10 8 11 12 8 4 5 5 5 9 10 8 10 10 13 12 6 4 7 2 4 3 2 290
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Citations to this article in year 2014 (12)

Title and authors Publication Year
HDL and cognition in neurodegenerative disorders
DA Hottman, D Chernick, S Cheng, Z Wang, L Li
Neurobiology of Disease 2014
Syndecan 4 Is Involved in Mediating HCV Entry through Interaction with Lipoviral Particle-Associated Apolipoprotein E
M Lefèvre, DJ Felmlee, M Parnot, TF Baumert, C Schuster
PloS one 2014
ApoE4 delays dendritic spine formation during neuron development and accelerates loss of mature spines in vitro
E Nwabuisi‑Heath, GW Rebeck, MJ LaDu, C Yu
ASN NEURO 2014
ApoA-I/HDL Generation and Intracellular Cholesterol Transport through Cytosolic Lipid-Protein Particles in Astrocytes
J Ito, M Michikawa
Journal of Lipids 2014
Macrophage-derived apoESendai suppresses atherosclerosis while causing lipoprotein glomerulopathy in hyperlipidemic mice
H Tavori, D Fan, I Giunzioni, L Zhu, MF Linton, AB Fogo, S Fazio
Journal of lipid research 2014
Development and partial characterisation of an antiserum against apolipoprotein B of the short-finned eel, Anguilla australis
EL Damsteegt, H Mizuta, Y Ozaki, N Hiramatsu, T Todo, A Hara, S Ijiri, S Adachi, PM Lokman
Journal of Comparative Physiology B 2014
Total apolipoprotein E levels and specific isoform composition in cerebrospinal fluid and plasma from Alzheimer’s disease patients and controls
E Martínez-Morillo, O Hansson, Y Atagi, G Bu, L Minthon, EP Diamandis, HM Nielsen
Acta Neuropathologica 2014
High-Density Lipoproteins and Cerebrovascular Integrity in Alzheimer’s Disease
S Stukas, J Robert, CL Wellington
Cell Metabolism 2014
Increasing cellular level of phosphatidic acid enhances FGF-1 production in long term-cultured rat astrocytes
Y Nagayasu, S Morita, H Hayashi, Y Miura, K Yokoyama, M Michikawa, J Ito
Brain Research 2014
Astrocyte׳s endogenous apoE generates HDL-like lipoproteins using previously synthesized cholesterol through interaction with ABCA1
J Ito, Y Nagayasu, Y Miura, S Yokoyama, M Michikawa
Brain Research 2014
HDL and cholesterol handling in the brain
C Vitali, CL Wellington, L Calabresi
Cardiovascular Research 2014
Syndecan 4 Is Involved in Mediating HCV Entry through Interaction with Lipoviral Particle-Associated Apolipoprotein E
M Lefèvre, DJ Felmlee, M Parnot, TF Baumert, C Schuster, R Ray
PloS one 2014

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