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Research Article Free access | 10.1172/JCI113053

Glucuronidation of 6 alpha-hydroxy bile acids by human liver microsomes.

A Radomińska-Pyrek, P Zimniak, Y M Irshaid, R Lester, T R Tephly, and J St Pyrek

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Published July 1, 1987 - More info

Published in Volume 80, Issue 1 on July 1, 1987
J Clin Invest. 1987;80(1):234–241. https://doi.org/10.1172/JCI113053.
© 1987 The American Society for Clinical Investigation
Published July 1, 1987 - Version history
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Abstract

The glucuronidation of 6-hydroxylated bile acids by human liver microsomes has been studied in vitro; for comparison, several major bile acids lacking a 6-hydroxyl group were also investigated. Glucuronidation rates for 6 alpha-hydroxylated bile acids were 10-20 times higher than those of substrates lacking a hydroxyl group in position 6. The highest rates measured were for hyodeoxy- and hyocholic acids, and kinetic analyses were carried out using these substrates. Rigorous product identification by high-field proton nuclear magnetic resonance and by electron impact mass spectrometry of methyl ester/peracetate derivatives revealed that 6-O-beta-D-glucuronides were the exclusive products formed in these enzymatic reactions. These results, together with literature data, indicate that 6 alpha-hydroxylation followed by 6-O-glucuronidation constitutes an alternative route of excretion of toxic hydrophobic bile acids.

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