Usage Information

Induction of terminal differentiation in human K562 erythroleukemia cells by arabinofuranosylcytosine.
C Luisi-DeLuca, T Mitchell, D Spriggs, D W Kufe
C Luisi-DeLuca, T Mitchell, D Spriggs, D W Kufe
View: Text | PDF
Research Article

Induction of terminal differentiation in human K562 erythroleukemia cells by arabinofuranosylcytosine.

Abstract

We have previously demonstrated that continuous exposure of human HL-60 human promyelocytes to 1-beta-D-arabinofuranosylcytosine (ara-C) results in the induction of terminal differentiation to monocyte-like cells. The present study extends these findings by demonstrating that ara-C induces hemoglobin synthesis in human K562 erythroleukemia cells. This effect occurs maximally at an ara-C concentration (5 X 10(-7) M) that results in K562 cytostasis. In contrast to the reversible effects of hemin and hydroxyurea on globin synthesis in this cell line, we have found that the induction of K562 hemoglobin synthesis by ara-C is irreversible. An induction of K562 hemoglobin synthesis also occurs with aphidicolin, another inhibitor of S-phase DNA synthesis, but not with vinblastine, an inhibitor of mitosis. Finally, ara-C induction of a differentiated K562 phenotype is accompanied by the loss of self-renewal capacity, a finding consistent with terminal differentiation.

Authors

C Luisi-DeLuca, T Mitchell, D Spriggs, D W Kufe

×

Usage data is cumulative from November 2025 through November 2026.

Usage JCI PMC
Text version 428 21
PDF 216 10
Figure 0 4
Scanned page 596 1
Citation downloads 248 0
Totals 1,488 36
Total Views 1,524
(Click and drag on plot area to zoom in. Click legend items above to toggle)

Usage information is collected from two different sources: this site (JCI) and Pubmed Central (PMC). JCI information (compiled daily) shows human readership based on methods we employ to screen out robotic usage. PMC information (aggregated monthly) is also similarly screened of robotic usage.

Various methods are used to distinguish robotic usage. For example, Google automatically scans articles to add to its search index and identifies itself as robotic; other services might not clearly identify themselves as robotic, or they are new or unknown as robotic. Because this activity can be misinterpreted as human readership, data may be re-processed periodically to reflect an improved understanding of robotic activity. Because of these factors, readers should consider usage information illustrative but subject to change.

Advertisement