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Philip W. Majerus, … , M. B. Smith, Virginia Minnich
Citation Information: J Clin Invest. 1971;50(8):1637-1643. https://doi.org/10.1172/JCI106652.
Philip W. Majerus, … , M. B. Smith, Virginia Minnich
Published August 1, 1971
Citation Information: J Clin Invest. 1971;50(8):1637-1643. https://doi.org/10.1172/JCI106652.
Abstract
The two enzymes required to synthesize glutathione de novo have been purified from human erythrocytes. Glutamylcysteine synthetase was purified 4300-fold and was approximately 80% pure based on polyacrylamide gel electrophoresis. The purified enzyme catalyzes the formation of 30.5 μmoles of γ-glutamyl-cysteine per mg of protein per hr and is inhibited by sulfhydryl inhibitors.
Authors
Philip W. Majerus, M. J. Brauner, M. B. Smith, Virginia Minnich
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