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Citations to this article

Mycobacterial growth inhibition is associated with trained innate immunity
Simone A. Joosten, … , Mihai G. Netea, Tom H.M. Ottenhoff
Simone A. Joosten, … , Mihai G. Netea, Tom H.M. Ottenhoff
Published February 20, 2018
Citation Information: J Clin Invest. 2018;128(5):1837-1851. https://doi.org/10.1172/JCI97508.
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Research Article Immunology Infectious disease Article has an altmetric score of 3

Mycobacterial growth inhibition is associated with trained innate immunity

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Abstract

The lack of defined correlates of protection hampers development of vaccines against tuberculosis (TB). In vitro mycobacterial outgrowth assays are thought to better capture the complexity of the human host/Mycobacterium tuberculosis (Mtb) interaction. Here, we used a mycobacterial growth inhibition assay (MGIA) based on peripheral blood mononuclear cells to investigate the capacity to control outgrowth of bacille Calmette-Guérin (BCG). Interestingly, strong control of BCG outgrowth was observed almost exclusively in individuals with recent exposure to Mtb, but not in (long-term) latent TB infection, and only modestly in BCG vaccinees. Mechanistically, control of mycobacterial outgrowth strongly correlated with the presence of a CD14dim monocyte population, but also required the presence of T cells. The nonclassical monocytes produced CXCL10, and CXCR3 receptor blockade inhibited the capacity to control BCG outgrowth. Expression of CXCR3 splice variants was altered in recently Mtb-exposed individuals. Cytokines previously associated with trained immunity were detected in MGIA supernatants, and CXCL9, CXCL10, and CXCL11 represent new markers of trained immunity. These data indicate that CXCR3 ligands are associated with trained immunity and are critical factors in controlling mycobacterial outgrowth. In conclusion, control of mycobacterial outgrowth early after exposure to Mtb is the result of trained immunity mediated by a CXCL10-producing nonclassical CD14dim monocyte subset.

Authors

Simone A. Joosten, Krista E. van Meijgaarden, Sandra M. Arend, Corine Prins, Fredrik Oftung, Gro Ellen Korsvold, Sandra V. Kik, Rob J.W. Arts, Reinout van Crevel, Mihai G. Netea, Tom H.M. Ottenhoff

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 Total
Citations: 9 7 12 12 23 13 15 3 94
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2020 (13)

Title and authors Publication Year
BCG vaccination in humans inhibits systemic inflammation in a sex-dependent manner
Valerie A. C. M. Koeken, L. Charlotte J. de Bree, Vera P. Mourits, Simone J.C.F.M. Moorlag, Jona Walk, Branko Cirovic, Rob J.W. Arts, Martin Jaeger, Helga Dijkstra, Heidi Lemmers, Leo A.B. Joosten, Christine Stabell Benn, Reinout van Crevel, Mihai Netea
Journal of Clinical Investigation 2020
Towards new TB vaccines
B Brazier, H McShane
Seminars in Immunopathology 2020
Cell-Mediated Immune Responses to in vivo-Expressed and Stage-Specific Mycobacterium tuberculosis Antigens in Latent and Active Tuberculosis Across Different Age Groups
M Coppola, R Villar-Hernández, KE van Meijgaarden, I Latorre, BM Moreno, E Garcia-Garcia, KL Franken, C Prat, Z Stojanovic, ML Galvão, JP Millet, J Sabriá, A Sánchez-Montalva, A Noguera-Julian, A Geluk, J Domínguez, TH Ottenhoff
Frontiers in immunology 2020
Tools for Assessing the Protective Efficacy of TB Vaccines in Humans: in vitro Mycobacterial Growth Inhibition Predicts Outcome of in vivo Mycobacterial Infection
R Tanner, I Satti, SA Harris, MK O'Shea, D Cizmeci, D O'Connor, A Chomka, M Matsumiya, R Wittenberg, AM Minassian, J Meyer, HA Fletcher, H McShane
Frontiers in immunology 2020
Lessons from Bacillus Calmette-Guérin: Harnessing Trained Immunity for Vaccine Development
ST Pasco, J Anguita
Cells 2020
Expanding the role of bacterial vaccines into life-course vaccination strategies and prevention of antimicrobial-resistant infections
JT Poolman
npj Vaccines 2020
Key recent advances in TB vaccine development and understanding of protective immune responses against Mycobacterium tuberculosis
TJ Scriba, MG Netea, AM Ginsberg
Seminars in Immunology 2020
Perspective for Precision Medicine for Tuberculosis
C Lange, R Aarnoutse, D Chesov, R van Crevel, SH Gillespie, HP Grobbel, B Kalsdorf, I Kontsevaya, A van Laarhoven, T Nishiguchi, A Mandalakas, M Merker, S Niemann, N Köhler, J Heyckendorf, M Reimann, M Ruhwald, P Sanchez-Carballo, D Schwudke, F Waldow, AR DiNardo
Frontiers in immunology 2020
Prevention of tuberculosis in macaques after intravenous BCG immunization
PA Darrah, JJ Zeppa, P Maiello, JA Hackney, MH Wadsworth, TK Hughes, S Pokkali, PA Swanson, NL Grant, MA Rodgers, M Kamath, CM Causgrove, DJ Laddy, A Bonavia, D Casimiro, PL Lin, E Klein, AG White, CA Scanga, AK Shalek, M Roederer, JA Flynn, RA Seder
Nature 2020
Neonatal BCG Vaccination Reduces Interferon-γ Responsiveness to Heterologous Pathogens in Infants From a Randomized Controlled Trial
B Freyne, NL Messina, S Donath, S Germano, R Bonnici, K Gardiner, D Casalaz, RM Robins-Browne, MG Netea, KL Flanagan, T Kollmann, N Curtis, V Abruzzo, K Allen, C Morrison, AL Ponsonby, P Vuillermin
The Journal of Infectious Diseases 2020
BCG vaccination strategy implemented to reduce the impact of COVID-19: Hype or Hope?
K Rajarshi, A Chatterjee, S Ray
2020
BCG as a game-changer to prevent the infection and severity of COVID-19 pandemic?
AR Sharma, G Batra, M Kumar, A Mishra, R Singla, A Singh, RS Singh, B Medhi
Allergologia et Immunopathologia 2020
Safety and COVID-19 Symptoms in Individuals Recently Vaccinated with BCG: a Retrospective Cohort Study
SJ Moorlag, RC van Deuren, CH van Werkhoven, M Jaeger, P Debisarun, E Taks, VP Mourits, VA Koeken, LC de Bree, T ten Doesschate, MC Cleophas, S Smeekens, M Oosting, FL van de Veerdonk, LA Joosten, J ten Oever, JW van der Meer, N Curtis, P Aaby, C Stabell-Benn, EJ Giamarellos-Bourboulis, M Bonten, R van Crevel, MG Netea
Cell reports. Medicine 2020

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