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Citations to this article

Prolonged human neural stem cell maturation supports recovery in injured rodent CNS
Paul Lu, … , Eileen Staufenberg, Mark H. Tuszynski
Paul Lu, … , Eileen Staufenberg, Mark H. Tuszynski
Published August 21, 2017
Citation Information: J Clin Invest. 2017;127(9):3287-3299. https://doi.org/10.1172/JCI92955.
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Research Article Neuroscience Article has an altmetric score of 80

Prolonged human neural stem cell maturation supports recovery in injured rodent CNS

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Abstract

Neural stem cells (NSCs) differentiate into both neurons and glia, and strategies using human NSCs have the potential to restore function following spinal cord injury (SCI). However, the time period of maturation for human NSCs in adult injured CNS is not well defined, posing fundamental questions about the design and implementation of NSC-based therapies. This work assessed human H9 NSCs that were implanted into sites of SCI in immunodeficient rats over a period of 1.5 years. Notably, grafts showed evidence of continued maturation over the entire assessment period. Markers of neuronal maturity were first expressed 3 months after grafting. However, neurogenesis, neuronal pruning, and neuronal enlargement continued over the next year, while total graft size remained stable over time. Axons emerged early from grafts in very high numbers, and half of these projections persisted by 1.5 years. Mature astrocyte markers first appeared after 6 months, while more mature oligodendrocyte markers were not present until 1 year after grafting. Astrocytes slowly migrated from grafts. Notably, functional recovery began more than 1 year after grafting. Thus, human NSCs retain an intrinsic human rate of maturation, despite implantation into the injured rodent spinal cord, yet they support delayed functional recovery, a finding of great importance in planning human clinical trials.

Authors

Paul Lu, Steven Ceto, Yaozhi Wang, Lori Graham, Di Wu, Hiromi Kumamaru, Eileen Staufenberg, Mark H. Tuszynski

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 Total
Citations: 3 1 6 13 11 5 12 8 3 62
Citation information
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Citations to this article in year 2023 (6)

Title and authors Publication Year
Engineered human spinal cord-like tissues with dorsal and ventral neuronal progenitors for spinal cord injury repair in rats and monkeys
Xu B, Liu D, Liu W, Long G, Liu W, Wu Y, He X, Shen Y, Jiang P, Yin M, Fan Y, Shen H, Shi L, Zhang Q, Xue W, Jin C, Chen Z, Chen B, Li J, Hu Y, Li X, Xiao Z, Zhao Y, Dai J
Bioactive Materials 2023
Gene expression and locomotor recovery in adult rats with spinal cord injury and plasma-synthesized polypyrrole/iodine application combined with a mixed rehabilitation scheme
Coyoy-Salgado A, Orozco-Barrios C, Sánchez-Torres S, Olayo MG, Cruz GJ, Morales-Corona J, Olayo R, Diaz-Ruiz A, Ríos C, Alvarez-Mejia L, Mondragón-Lozano R, Morales-Guadarrama A, Alonso-García AL, Fabela-Sánchez O, Salgado-Ceballos H
Frontiers in neurology 2023
Moving CNS axon growth and regeneration research into human model systems
Lear BP, Moore DL
Frontiers in neuroscience 2023
Transplanting Human Neural Stem Cells with ≈50% Reduction of SOX9 Gene Dosage Promotes Tissue Repair and Functional Recovery from Severe Spinal Cord Injury
Liu JA, Tam KW, Chen YL, Feng X, Chan CW, Lo AL, Wu KL, Hui M, Wu M, Chan KK, Cheung MP, Cheung CW, Shum DK, Chan Y, Cheung M
Advanced Science 2023
Preclinical long-term safety of intraspinal transplantation of human dorsal spinal GABA neural progenitor cells
Zheng X, Liu Z, He Z, Xu J, Wang Y, Gong C, Zhang R, Zhang SC, Chen H, Wang W
iScience 2023
Electrical stimulation affects the differentiation of transplanted regionally specific human spinal neural progenitor cells (sNPCs) after chronic spinal cord injury
Patil N, Korenfeld O, Scalf RN, Lavoie N, Huntemer-Silveira A, Han G, Swenson R, Parr AM
Stem Cell Research & Therapy 2023

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ISSN: 0021-9738 (print), 1558-8238 (online)

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