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Citations to this article

TGF-β/β2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome
Jian Chen, … , Hidekazu Tsukamoto, Lopa Mishra
Jian Chen, … , Hidekazu Tsukamoto, Lopa Mishra
Published January 19, 2016
Citation Information: J Clin Invest. 2016;126(2):527-542. https://doi.org/10.1172/JCI80937.
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Research Article Article has an altmetric score of 2

TGF-β/β2-spectrin/CTCF-regulated tumor suppression in human stem cell disorder Beckwith-Wiedemann syndrome

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Abstract

Beckwith-Wiedemann syndrome (BWS) is a human stem cell disorder, and individuals with this disease have a substantially increased risk (~800-fold) of developing tumors. Epigenetic silencing of β2-spectrin (β2SP, encoded by SPTBN1), a SMAD adaptor for TGF-β signaling, is causally associated with BWS; however, a role of TGF-β deficiency in BWS-associated neoplastic transformation is unexplored. Here, we have reported that double-heterozygous Sptbn1+/– Smad3+/– mice, which have defective TGF-β signaling, develop multiple tumors that are phenotypically similar to those of BWS patients. Moreover, tumorigenesis-associated genes IGF2 and telomerase reverse transcriptase (TERT) were overexpressed in fibroblasts from BWS patients and TGF-β–defective mice. We further determined that chromatin insulator CCCTC-binding factor (CTCF) is TGF-β inducible and facilitates TGF-β–mediated repression of TERT transcription via interactions with β2SP and SMAD3. This regulation was abrogated in TGF-β–defective mice and BWS, resulting in TERT overexpression. Imprinting of the IGF2/H19 locus and the CDKN1C/KCNQ1 locus on chromosome 11p15.5 is mediated by CTCF, and this regulation is lost in BWS, leading to aberrant overexpression of growth-promoting genes. Therefore, we propose that loss of CTCF-dependent imprinting of tumor-promoting genes, such as IGF2 and TERT, results from a defective TGF-β pathway and is responsible at least in part for BWS-associated tumorigenesis as well as sporadic human cancers that are frequently associated with SPTBN1 and SMAD3 mutations.

Authors

Jian Chen, Zhi-Xing Yao, Jiun-Sheng Chen, Young Jin Gi, Nina M. Muñoz, Suchin Kundra, H. Franklin Herlong, Yun Seong Jeong, Alexei Goltsov, Kazufumi Ohshiro, Nipun A. Mistry, Jianping Zhang, Xiaoping Su, Sanaa Choufani, Abhisek Mitra, Shulin Li, Bibhuti Mishra, Jon White, Asif Rashid, Alan Yaoqi Wang, Milind Javle, Marta Davila, Peter Michaely, Rosanna Weksberg, Wayne L. Hofstetter, Milton J. Finegold, Jerry W. Shay, Keigo Machida, Hidekazu Tsukamoto, Lopa Mishra

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 Total
Citations: 2 2 7 3 5 2 2 3 3 3 32
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Citations to this article in year 2018 (3)

Title and authors Publication Year
A Pan-Cancer Analysis Reveals High-Frequency Genetic Alterations in Mediators of Signaling by the TGF-β Superfamily
A Korkut, S Zaidi, RS Kanchi, S Rao, NR Gough, A Schultz, X Li, PL Lorenzi, AC Berger, G Robertson, LN Kwong, M Datto, J Roszik, S Ling, V Ravikumar, G Manyam, A Rao, S Shelley, Y Liu, Z Ju, D Hansel, G de Velasco, A Pennathur, JB Andersen, CJ O'Rourke, K Ohshiro, W Jogunoori, BN Nguyen, S Li, HU Osmanbeyoglu, JA Ajani, SA Mani, A Houseman, M Wiznerowicz, J Chen, S Gu, W Ma, J Zhang, P Tong, AD Cherniack, C Deng, L Resar, JN Weinstein, L Mishra, R Akbani, A Blum, SJ Caesar-Johnson, JA Demchok, I Felau, M Kasapi, ML Ferguson, CM Hutter, HJ Sofia, R Tarnuzzer, P Wang, Z Wang, L Yang, JC Zenklusen, JJ Zhang, S Chudamani, J Liu, L Lolla, R Naresh, T Pihl, Q Sun, Y Wan, Y Wu, J Cho, T DeFreitas, S Frazer, N Gehlenborg, G Getz, DI Heiman, J Kim, MS Lawrence, P Lin, S Meier, MS Noble, G Saksena, D Voet, H Zhang, B Bernard, N Chambwe, V Dhankani, T Knijnenburg, R Kramer, K Leinonen, Y Liu, M Miller, S Reynolds, I Shmulevich, V Thorsson, W Zhang, R Akbani, BM Broom, AM Hegde, Z Ju, RS Kanchi, A Korkut, J Li, H Liang, S Ling, W Liu, Y Lu, GB Mills, KS Ng, A Rao, M Ryan, J Wang, JN Weinstein, J Zhang, A Abeshouse, J Armenia, D Chakravarty, WK Chatila, I de Bruijn, J Gao, BE Gross, ZJ Heins, R Kundra, K La, M Ladanyi, A Luna, MG Nissan, A Ochoa, SM Phillips, E Reznik, F Sanchez-Vega, C Sander, N Schultz, R Sheridan, SO Sumer, Y Sun, BS Taylor, J Wang, H Zhang, P Anur, M Peto, P Spellman, C Benz, JM Stuart, CK Wong, C Yau, DN Hayes, JS Parker, MD Wilkerson, A Ally, M Balasundaram, R Bowlby, D Brooks, R Carlsen, E Chuah, N Dhalla, R Holt, SJ Jones, K Kasaian, D Lee, Y Ma, MA Marra, M Mayo, RA Moore, AJ Mungall, K Mungall, AG Robertson, S Sadeghi, JE Schein, P Sipahimalani, A Tam, N Thiessen, K Tse, T Wong, AC Berger, R Beroukhim, AD Cherniack, C Cibulskis, SB Gabriel, GF Gao, G Ha, M Meyerson, SE Schumacher, J Shih, MH Kucherlapati, RS Kucherlapati, S Baylin, L Cope, L Danilova, MS Bootwalla, PH Lai, DT Maglinte, DJ Berg, DJ Weisenberger, JT Auman, S Balu, T Bodenheimer, C Fan, KA 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Silveira, DO Vidal, A Burnette, J Eschbacher, B Hermes, A Noss, R Singh, ML Anderson, PD Castro, M Ittmann, D Huntsman, B Kohl, X Le, R Thorp, C Andry, ER Duffy, V Lyadov, O Paklina, G Setdikova, A Shabunin, M Tavobilov, C McPherson, R Warnick, R Berkowitz, D Cramer, C Feltmate, N Horowitz, A Kibel, M Muto, CP Raut, A Malykh, JS Barnholtz-Sloan, W Barrett, K Devine, J Fulop, QT Ostrom, K Shimmel, Y Wolinsky, AE Sloan, AD Rose, F Giuliante, M Goodman, BY Karlan, CH Hagedorn, J Eckman, J Harr, J Myers, K Tucker, LA Zach, B Deyarmin, H Hu, L Kvecher, C Larson, RJ Mural, S Somiari, A Vicha, T Zelinka, J Bennett, M Iacocca, B Rabeno, P Swanson, M Latour, L Lacombe, B Têtu, A Bergeron, M McGraw, SM Staugaitis, J Chabot, H Hibshoosh, A Sepulveda, T Su, T Wang, O Potapova, O Voronina, L Desjardins, O Mariani, S Roman-Roman, X Sastre, MH Stern, F Cheng, S Signoretti, A Berchuck, D Bigner, E Lipp, J Marks, S McCall, R McLendon, A Secord, A Sharp, M Behera, DJ Brat, A Chen, K Delman, S Force, F 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G Petersen, F Que, M Rivera, L Roberts, R Smallridge, T Smyrk, M Stanton, RH Thompson, M Torbenson, JD Yang, L Zhang, F Brimo, JA Ajani, AM Gonzalez, C Behrens, J Bondaruk, R Broaddus, B Czerniak, B Esmaeli, J Fujimoto, J Gershenwald, C Guo, AJ Lazar, C Logothetis, F Meric-Bernstam, C Moran, L Ramondetta, D Rice, A Sood, P Tamboli, T Thompson, P Troncoso, A Tsao, I Wistuba, C Carter, L Haydu, P Hersey, V Jakrot, H Kakavand, R Kefford, K Lee, G Long, G Mann, M Quinn, R Saw, R Scolyer, K Shannon, A Spillane, J Stretch, M Synott, J Thompson, J Wilmott, H Al-Ahmadie, TA Chan, R Ghossein, A Gopalan, DA Levine, V Reuter, S Singer, B Singh, NV Tien, T Broudy, C Mirsaidi, P Nair, P Drwiega, J Miller, J Smith, H Zaren, JW Park, NP Hung, E Kebebew, WM Linehan, AR Metwalli, K Pacak, PA Pinto, M Schiffman, LS Schmidt, CD Vocke, N Wentzensen, R Worrell, H Yang, M Moncrieff, C Goparaju, J Melamed, H Pass, N Botnariuc, I Caraman, M Cernat, I Chemencedji, A Clipca, S Doruc, G Gorincioi, S Mura, M 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