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Citations to this article

Differential coreceptor expression allows for independent evolution of non–syncytium-inducing and syncytium-inducing HIV-1
Ronald P. van Rij, … , Ana-Maria de Roda Husman, Hanneke Schuitemaker
Ronald P. van Rij, … , Ana-Maria de Roda Husman, Hanneke Schuitemaker
Published October 15, 2000
Citation Information: J Clin Invest. 2000;106(8):1039-1052. https://doi.org/10.1172/JCI7953.
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Article Article has an altmetric score of 3

Differential coreceptor expression allows for independent evolution of non–syncytium-inducing and syncytium-inducing HIV-1

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Abstract

We demonstrated previously that CD45RA+ CD4+ T cells are infected primarily by syncytium-inducing (SI) HIV-1 variants, whereas CD45RO+ CD4+ T cells harbor both non-SI (NSI) and SI HIV-1 variants. Here, we studied evolution of tropism for CD45RA+ and CD45RO+ CD4+ cells, coreceptor usage, and molecular phylogeny of coexisting NSI and SI HIV-1 clones that were isolated from four patients in the period spanning SI conversion. NSI variants were CCR5-restricted and could be isolated throughout infection from CD45RO+ CD4+ cells. SI variants seemed to evolve in CD45RO+ CD4+ cells, but, in time, SI HIV-1 infection of CD45RA+ CD4+ cells equaled infection of CD45RO+ CD4+ cells. In parallel with this shift, SI HIV-1 variants first used both coreceptors CCR5 and CXCR4, but eventually lost the ability to use CCR5. Phylogenetically, NSI and SI HIV-1 populations diverged over time. We observed a differential expression of HIV-1 coreceptors within CD45RA+ and CD45RO+ cells, which allowed us to isolate virus from purified CCR5+ CXCR4– and CCR5– CXCR4+ CD4+ cells. The CCR5+ subset was exclusively infected by CCR5-dependent HIV-1 clones, whereas SI clones were preferentially isolated from the CXCR4+ subset. The differential expression of HIV-1 coreceptors provides distinct cellular niches for NSI and SI HIV-1, contributing to their coexistence and independent evolutionary pathways.

Authors

Ronald P. van Rij, Hetty Blaak, Janny A. Visser, Margreet Brouwer, Ronald Rientsma, Silvia Broersen, Ana-Maria de Roda Husman, Hanneke Schuitemaker

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Total citations by year

Year: 2025 2022 2020 2017 2015 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 Total
Citations: 1 1 1 1 1 1 2 6 3 4 5 5 9 6 6 7 5 4 68
Citation information
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Citations to this article in year 2008 (5)

Title and authors Publication Year
Role of HIV Gp41 mediated fusion/hemifusion in bystander apoptosis
H Garg, R Blumenthal
Cellular and molecular life sciences : CMLS 2008
Bioinformatic prediction programs underestimate the frequency of CXCR4 usage by R5X4 HIV type 1 in brain and other tissues
ME Mefford, PR Gorry, K Kunstman, SM Wolinsky, D Gabuzda
AIDS Research and Human Retroviruses 2008
Viral and Host Determinants of HIV-1 Disease Progression
H Schuitemaker, AB Wout
Global HIV/AIDS Medicine 2008
Dendritic Cells Preferentially Transfer CXCR4-Using Human Immunodeficiency Virus Type 1 Variants to CD4 + T Lymphocytes in trans
T van Montfort, AA Thomas, G Pollakis, WA Paxton
Journal of virology 2008
Evolution of CCR5 Use before and during Coreceptor Switching
M Coetzer, R Nedellec, J Salkowitz, S McLaughlin, Y Liu, L Heath, JI Mullins, DE Mosier
Journal of virology 2008

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Referenced in 5 patents
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