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Citations to this article

IQGAP1-dependent scaffold suppresses RhoA and inhibits airway smooth muscle contraction
Mallar Bhattacharya, … , Xiaozhu Huang, Dean Sheppard
Mallar Bhattacharya, … , Xiaozhu Huang, Dean Sheppard
Published October 1, 2014
Citation Information: J Clin Invest. 2014;124(11):4895-4898. https://doi.org/10.1172/JCI76658.
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Brief Report Pulmonology Article has an altmetric score of 19

IQGAP1-dependent scaffold suppresses RhoA and inhibits airway smooth muscle contraction

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Abstract

The intracellular scaffold protein IQGAP1 supports protein complexes in conjunction with numerous binding partners involved in multiple cellular processes. Here, we determined that IQGAP1 modulates airway smooth muscle contractility. Compared with WT controls, at baseline as well as after immune sensitization and challenge, Iqgap1–/– mice had higher airway responsiveness. Tracheal rings from Iqgap1–/– mice generated greater agonist-induced contractile force, even after removal of the epithelium. RhoA, a regulator of airway smooth muscle contractility, was activated in airway smooth muscle lysates from Iqgap1–/– mice. Likewise, knockdown of IQGAP1 in primary human airway smooth muscle cells increased RhoA activity. Immunoprecipitation studies indicated that IQGAP1 binds to both RhoA and p190A-RhoGAP, a GTPase-activating protein that normally inhibits RhoA activation. Proximity ligation assays in primary airway human smooth muscle cells and mouse tracheal sections revealed colocalization of p190A-RhoGAP and RhoA; however, these proteins did not colocalize in IQGAP1 knockdown cells or in Iqgap1–/– trachea. Compared with healthy controls, human subjects with asthma had decreased IQGAP1 expression in airway biopsies. Together, these data demonstrate that IQGAP1 acts as a scaffold that colocalizes p190A-RhoGAP and RhoA, inactivating RhoA and suppressing airway smooth muscle contraction. Furthermore, our results suggest that IQGAP1 has the potential to modulate airway contraction severity in acute asthma.

Authors

Mallar Bhattacharya, Aparna Sundaram, Makoto Kudo, Jessica Farmer, Previn Ganesan, Amin Khalifeh-Soltani, Mehrdad Arjomandi, Kamran Atabai, Xiaozhu Huang, Dean Sheppard

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Total citations by year

Year: 2024 2022 2021 2020 2019 2018 2016 2015 Total
Citations: 1 2 2 1 5 1 3 2 17
Citation information
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Citations to this article in year 2021 (2)

Title and authors Publication Year
RhoA and Cdc42 in T cells: Are they targetable for T cell-mediated inflammatory diseases?
F Guo
2021
Selectivity Determinants of RHO GTPase Binding to IQGAPs
N Mosaddeghzadeh, K Nouri, OH Krumbach, E Amin, R Dvorsky, MR Ahmadian
International journal of molecular sciences 2021

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