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Citations to this article

Regulation of pancreatic PC1 and PC2 associated with increased glucagon-like peptide 1 in diabetic rats
Ying Nie, … , Daniel Pipeleers, Theodore C. Friedman
Ying Nie, … , Daniel Pipeleers, Theodore C. Friedman
Published April 1, 2000
Citation Information: J Clin Invest. 2000;105(7):955-965. https://doi.org/10.1172/JCI7456.
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Article

Regulation of pancreatic PC1 and PC2 associated with increased glucagon-like peptide 1 in diabetic rats

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Abstract

The pancreatic processing enzymes, PC1 and PC2, convert proinsulin to insulin and convert proglucagon to glucagon and glucagon-like peptide 1 (GLP-1). We examined the effect of streptozotocin (STZ) treatment on the regulation of these enzymes and the production of insulin, glucagon, and GLP-1 in the rat. Pancreatic PC1 and PC2 mRNA increased >2-fold and >4-fold, respectively, in rats receiving intraperitoneal STZ (50 mg/kg) daily for 5 days. Immunocytochemistry revealed that, although pancreatic islet cells in the STZ-treated rats were sparse and atrophic PC1, PC2, glucagon, and GLP-1 immunoreactivity increased dramatically in the remaining islet cells. Heightened PC1 and PC2 expression was seen in cells expressing glucagon but not in insulin-expressing cells. Furthermore, in STZ-treated rats, bioactive GLP-17–36 amide accumulated in pancreatic extracts and serum 3- and 2.5-fold, respectively, over control animals. This treatment also caused a 2-fold increase in the ratio of amidated forms of GLP-1 immunoreactivity to total glucagon immunoreactivity in the pancreas but did not affect the ratio of proinsulin to insulin. We conclude that hyperglycemic rats have an increased expression of prohormone converting enzymes in islet α cells, leading to an increase in amidated GLP-1, which can then exert an insulinotropic effect on the remaining β cells.

Authors

Ying Nie, Masahiro Nakashima, Patricia L. Brubaker, Qiao-Ling Li, Riccardo Perfetti, Erik Jansen, Yasmeen Zambre, Daniel Pipeleers, Theodore C. Friedman

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 2002 2001 Total
Citations: 1 5 2 6 6 11 7 6 9 8 7 17 6 2 115 28 8 1 4 2 4 5 9 2 2 273
Citation information
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Citations to this article in year 2012 (2)

Title and authors Publication Year
α-cell role in β-cell generation and regeneration
JF Habener, V Stanojevic
Islets 2012
INCRETIN-BASED THERAPY
Y Fujitani
Juntendo Medical Journal 2012

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