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Citations to this article

Rice-based oral antibody fragment prophylaxis and therapy against rotavirus infection
Daisuke Tokuhara, … , Lennart Hammarström, Yoshikazu Yuki
Daisuke Tokuhara, … , Lennart Hammarström, Yoshikazu Yuki
Published August 8, 2013
Citation Information: J Clin Invest. 2013;123(9):3829-3838. https://doi.org/10.1172/JCI70266.
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Research Article Infectious disease Article has an altmetric score of 164

Rice-based oral antibody fragment prophylaxis and therapy against rotavirus infection

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Abstract

Rotavirus-induced diarrhea is a life-threatening disease in immunocompromised individuals and in children in developing countries. We have developed a system for prophylaxis and therapy against rotavirus disease using transgenic rice expressing the neutralizing variable domain of a rotavirus-specific llama heavy-chain antibody fragment (MucoRice-ARP1). MucoRice-ARP1 was produced at high levels in rice seeds using an overexpression system and RNAi technology to suppress the production of major rice endogenous storage proteins. Orally administered MucoRice-ARP1 markedly decreased the viral load in immunocompetent and immunodeficient mice. The antibody retained in vitro neutralizing activity after long-term storage (>1 yr) and boiling and conferred protection in mice even after heat treatment at 94°C for 30 minutes. High-yield, water-soluble, and purification-free MucoRice-ARP1 thus forms the basis for orally administered prophylaxis and therapy against rotavirus infections.

Authors

Daisuke Tokuhara, Beatriz Álvarez, Mio Mejima, Tomoko Hiroiwa, Yuko Takahashi, Shiho Kurokawa, Masaharu Kuroda, Masaaki Oyama, Hiroko Kozuka-Hata, Tomonori Nochi, Hiroshi Sagara, Farah Aladin, Harold Marcotte, Leon G.J. Frenken, Miren Iturriza-Gómara, Hiroshi Kiyono, Lennart Hammarström, Yoshikazu Yuki

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Total citations by year

Year: 2024 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 Total
Citations: 3 3 3 2 4 2 3 6 6 3 3 38
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2013 (3)

Title and authors Publication Year
RNAi-mediated suppression of endogenous storage proteins leads to a change in localization of overexpressed cholera toxin B-subunit and the allergen protein RAG2 in rice seeds
S Kurokawa, M Kuroda, M Mejima, R Nakamura, Y Takahashi, H Sagara, N Takeyama, S Satoh, H Kiyono, R Teshima, T Masumura, Y Yuki
Plant Cell Reports 2013
Rice-derived rotavirus antibody shows promise
MT Tse
Nature Reviews Drug Discovery 2013
Rice yields new oral therapy for rotavirus infection
K Smith
Nature Reviews Gastroenterology & Hepatology 2013

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