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Citations to this article

Parthenogenetic stem cells for tissue-engineered heart repair
Michael Didié, … , Loren J. Field, Wolfram-Hubertus Zimmermann
Michael Didié, … , Loren J. Field, Wolfram-Hubertus Zimmermann
Published February 22, 2013
Citation Information: J Clin Invest. 2013;123(3):1285-1298. https://doi.org/10.1172/JCI66854.
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Technical Advance Article has an altmetric score of 58

Parthenogenetic stem cells for tissue-engineered heart repair

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Abstract

Uniparental parthenotes are considered an unwanted byproduct of in vitro fertilization. In utero parthenote development is severely compromised by defective organogenesis and in particular by defective cardiogenesis. Although developmentally compromised, apparently pluripotent stem cells can be derived from parthenogenetic blastocysts. Here we hypothesized that nonembryonic parthenogenetic stem cells (PSCs) can be directed toward the cardiac lineage and applied to tissue-engineered heart repair. We first confirmed similar fundamental properties in murine PSCs and embryonic stem cells (ESCs), despite notable differences in genetic (allelic variability) and epigenetic (differential imprinting) characteristics. Haploidentity of major histocompatibility complexes (MHCs) in PSCs is particularly attractive for allogeneic cell-based therapies. Accordingly, we confirmed acceptance of PSCs in MHC-matched allotransplantation. Cardiomyocyte derivation from PSCs and ESCs was equally effective. The use of cardiomyocyte-restricted GFP enabled cell sorting and documentation of advanced structural and functional maturation in vitro and in vivo. This included seamless electrical integration of PSC-derived cardiomyocytes into recipient myocardium. Finally, we enriched cardiomyocytes to facilitate engineering of force-generating myocardium and demonstrated the utility of this technique in enhancing regional myocardial function after myocardial infarction. Collectively, our data demonstrate pluripotency, with unrestricted cardiogenicity in PSCs, and introduce this unique cell type as an attractive source for tissue-engineered heart repair.

Authors

Michael Didié, Peter Christalla, Michael Rubart, Vijayakumar Muppala, Stephan Döker, Bernhard Unsöld, Ali El-Armouche, Thomas Rau, Thomas Eschenhagen, Alexander P. Schwoerer, Heimo Ehmke, Udo Schumacher, Sigrid Fuchs, Claudia Lange, Alexander Becker, Wen Tao, John A. Scherschel, Mark H. Soonpaa, Tao Yang, Qiong Lin, Martin Zenke, Dong-Wook Han, Hans R. Schöler, Cornelia Rudolph, Doris Steinemann, Brigitte Schlegelberger, Steve Kattman, Alec Witty, Gordon Keller, Loren J. Field, Wolfram-Hubertus Zimmermann

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Total citations by year

Year: 2025 2024 2023 2021 2020 2019 2018 2017 2016 2015 2014 2013 2004 Total
Citations: 1 1 1 6 1 3 2 10 5 5 13 9 1 58
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2017 (10)

Title and authors Publication Year
Immunological Properties of Murine Parthenogenetic Stem Cells and Their Differentiation Products
H Johannsen, V Muppala, C Gröschel, S Monecke, L Elsner, M Didié, WH Zimmermann, R Dressel
Frontiers in immunology 2017
Myocardial Tissue Engineering for Regenerative Applications
B Fujita, WH Zimmermann
Current Cardiology Reports 2017
Immunological Properties of Murine Parthenogenetic Stem Cell-Derived Cardiomyocytes and Engineered Heart Muscle
M Didié, S Galla, V Muppala, R Dressel, WH Zimmermann
Frontiers in immunology 2017
Mouse Parthenogenetic Embryonic Stem Cells with Biparental-Like Expression of Imprinted Genes Generate Cortical-Like Neurons That Integrate into the Injured Adult Cerebral Cortex: Cerebral Cortex from Parthenogenetic ESCs
A Varrault, S Eckardt, B Girard, AL Digarcher, I Sassetti, C Meusnier, C Ripoll, A Badalyan, F Bertaso, KJ McLaughlin, L Journot, T Bouschet
Stem Cells 2017
Efficient Killing of Murine Pluripotent Stem Cells by Natural Killer (NK) Cells Requires Activation by Cytokines and Partly Depends on the Activating NK Receptor NKG2D
C Gröschel, D Hübscher, J Nolte, S Monecke, A Sasse, L Elsner, W Paulus, C Trenkwalder, B Polić, A Mansouri, K Guan, R Dressel
Frontiers in immunology 2017
Remuscularization of the failing heart: Engineered heart repair
WH Zimmermann
The Journal of Physiology 2017
Engineered Heart Repair
B Fujita, WH Zimmermann
Clinical Pharmacology & Therapeutics 2017
Translating Myocardial Remuscularization
WH Zimmermann
Circulation research 2017
Stammzellbasierte kardiale Regeneration nach Infarkt
M Reinsch, F Weinberger
Herz 2017
Alterations of protein glycosylation in embryonic stem cells during adipogenesis
W Liu, X Yan, W Liu, Y Wang, Y Rao, H Yu, J Cui, X Xie, M Sun, L Yin, H Li, F Chen
International journal of molecular medicine 2017

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