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Citations to this article

Improved detection suggests all Merkel cell carcinomas harbor Merkel polyomavirus
Scott J. Rodig, … , Linda C. Wang, James A. DeCaprio
Scott J. Rodig, … , Linda C. Wang, James A. DeCaprio
Published November 1, 2012
Citation Information: J Clin Invest. 2012;122(12):4645-4653. https://doi.org/10.1172/JCI64116.
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Research Article Oncology Article has an altmetric score of 22

Improved detection suggests all Merkel cell carcinomas harbor Merkel polyomavirus

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Abstract

A human polyomavirus was recently discovered in Merkel cell carcinoma (MCC) specimens. The Merkel cell polyomavirus (MCPyV) genome undergoes clonal integration into the host cell chromosomes of MCC tumors and expresses small T antigen and truncated large T antigen. Previous studies have consistently reported that MCPyV can be detected in approximately 80% of all MCC tumors. We sought to increase the sensitivity of detection of MCPyV in MCC by developing antibodies capable of detecting large T antigen by immunohistochemistry. In addition, we expanded the repertoire of quantitative PCR primers specific for MCPyV to improve the detection of viral DNA in MCC. Here we report that a novel monoclonal antibody detected MCPyV large T antigen expression in 56 of 58 (97%) unique MCC tumors. PCR analysis specifically detected viral DNA in all 60 unique MCC tumors tested. We also detected inactivating point substitution mutations of TP53 in the two MCC specimens that lacked large T antigen expression and in only 1 of 56 tumors positive for large T antigen. These results indicate that MCPyV is present in MCC tumors more frequently than previously reported and that mutations in TP53 tend to occur in MCC tumors that fail to express MCPyV large T antigen.

Authors

Scott J. Rodig, Jingwei Cheng, Jacek Wardzala, Andrew DoRosario, Jessica J. Scanlon, Alvaro C. Laga, Alejandro Martinez-Fernandez, Justine A. Barletta, Andrew M. Bellizzi, Subhashini Sadasivam, Dustin T. Holloway, Dylan J. Cooper, Thomas S. Kupper, Linda C. Wang, James A. DeCaprio

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2009 Total
Citations: 4 4 4 6 6 8 1 7 13 10 6 14 12 1 96
Citation information
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Citations to this article in year 2013 (12)

Title and authors Publication Year
Merkel polyomavirus-specific T cells fluctuate with merkel cell carcinoma burden and express therapeutically targetable PD-1 and Tim-3 exhaustion markers
OK Afanasiev, L Yelistratova, N Miller, K Nagase, K Paulson, JG Iyer, D Ibrani, DM Koelle, P Nghiem
Clinical cancer research 2013
Tumor-specific T cells in human Merkel cell carcinomas: a possible role for Tregs and T cell exhaustion in reducing T cell responses
M Dowlatshahi, V Huang, A Gehad, Y Jiang, A Calarese, JE Teague, A Dorosario, J Cheng, P Nghiem, C Schanbacher, M Thakuria, C Schmults, LC Wang, RA Clark
Journal of Investigative Dermatology 2013
Regression of Metastatic Merkel Cell Carcinoma Following Transfer of Polyomavirus-Specific T Cells and Therapies Capable of Reinducing HLA Class-I
AG Chapuis, OK Afanasiev, JG Iyer, KG Paulson, U Parvathaneni, JH Hwang, I Lai, IM Roberts, HL Sloan, S Bhatia, KC Shibuya, T Gooley, C Desmarais, DM Koelle, C Yee, P Nghiem
Cancer immunology research 2013
A cornucopia of human polyomaviruses
JA DeCaprio, RL Garcea
Nature Reviews Microbiology 2013
Merkel cell polyomavirus large T antigen disrupts host genomic integrity and inhibits cellular proliferation
J Li, X Wang, J Diaz, SH Tsang, CB Buck, J You
Journal of virology 2013
Merkel cell polyomavirus large T antigen has growth-promoting and inhibitory activities
J Cheng, O Rozenblatt-Rosen, KG Paulson, P Nghiem, JA DeCaprio
Journal of virology 2013
MicroRNA Expression Patterns Related to Merkel Cell Polyomavirus Infection in Human Merkel Cell Carcinoma
H Xie, L Lee, S Caramuta, A Höög, N Browaldh, V Björnhagen, C Larsson, WO Lui
Journal of Investigative Dermatology 2013
Different serologic behavior of MCPyV, TSPyV, HPyV6, HPyV7 and HPyV9 polyomaviruses found on the skin
E der Meijden, S Bialasiewicz, RJ Rockett, SJ Tozer, TP Sloots, MC Feltkamp
PloS one 2013
Merkel cell polyomavirus and extrapulmonary small cell carcinoma
KC Hourdequin, JA Lefferts, JB Brennick, MS Ernstoff, GJ Tsongalis, JM Pipas
Oncology Letters 2013
Emerging and mechanism-based therapies for recurrent or metastatic Merkel cell carcinoma
NJ Miller, S Bhatia, U Parvathaneni, JG Iyer, P Nghiem
Current Treatment Options in Oncology 2013
Palliative treatment for in-transit cutaneous metastases of Merkel cell carcinoma using surface-mold computer-optimized high-dose-rate brachytherapy
L Garibyan, SE Cotter, JL Hansen, C Noell, A Dorosario, DA O’Farrell, PM Devlin, LC Wang
The Cancer Journal 2013
PD-L1 Expression in the Merkel Cell Carcinoma Microenvironment: Association with Inflammation, Merkel Cell Polyomavirus, and Overall Survival
EJ Lipson, JG Vincent, M Loyo, LT Kagohara, BS Luber, H Wang, H Xu, SK Nayar, TS Wang, D Sidransky, RA Anders, SL Topalian, JM Taube
Cancer immunology research 2013

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