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Citations to this article

Improved detection suggests all Merkel cell carcinomas harbor Merkel polyomavirus
Scott J. Rodig, … , Linda C. Wang, James A. DeCaprio
Scott J. Rodig, … , Linda C. Wang, James A. DeCaprio
Published November 1, 2012
Citation Information: J Clin Invest. 2012;122(12):4645-4653. https://doi.org/10.1172/JCI64116.
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Research Article Oncology Article has an altmetric score of 22

Improved detection suggests all Merkel cell carcinomas harbor Merkel polyomavirus

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Abstract

A human polyomavirus was recently discovered in Merkel cell carcinoma (MCC) specimens. The Merkel cell polyomavirus (MCPyV) genome undergoes clonal integration into the host cell chromosomes of MCC tumors and expresses small T antigen and truncated large T antigen. Previous studies have consistently reported that MCPyV can be detected in approximately 80% of all MCC tumors. We sought to increase the sensitivity of detection of MCPyV in MCC by developing antibodies capable of detecting large T antigen by immunohistochemistry. In addition, we expanded the repertoire of quantitative PCR primers specific for MCPyV to improve the detection of viral DNA in MCC. Here we report that a novel monoclonal antibody detected MCPyV large T antigen expression in 56 of 58 (97%) unique MCC tumors. PCR analysis specifically detected viral DNA in all 60 unique MCC tumors tested. We also detected inactivating point substitution mutations of TP53 in the two MCC specimens that lacked large T antigen expression and in only 1 of 56 tumors positive for large T antigen. These results indicate that MCPyV is present in MCC tumors more frequently than previously reported and that mutations in TP53 tend to occur in MCC tumors that fail to express MCPyV large T antigen.

Authors

Scott J. Rodig, Jingwei Cheng, Jacek Wardzala, Andrew DoRosario, Jessica J. Scanlon, Alvaro C. Laga, Alejandro Martinez-Fernandez, Justine A. Barletta, Andrew M. Bellizzi, Subhashini Sadasivam, Dustin T. Holloway, Dylan J. Cooper, Thomas S. Kupper, Linda C. Wang, James A. DeCaprio

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2009 Total
Citations: 4 4 4 6 6 8 1 7 13 10 6 14 12 1 96
Citation information
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Citations to this article in year 2020 (8)

Title and authors Publication Year
Clinical and molecular characterization of virus-positive and virus-negative Merkel cell carcinoma
GJ Starrett, M Thakuria, T Chen, C Marcelus, J Cheng, J Nomburg, AR Thorner, MK Slevin, W Powers, RT Burns, C Perry, A Piris, FC Kuo, G Rabinowits, A Giobbie-Hurder, LE MacConaill, JA DeCaprio
Genome Medicine 2020
Merkel Cell Polyomavirus and Merkel Cell Carcinoma
V Pietropaolo, C Prezioso, U Moens
Cancers 2020
Molecular Mechanisms of Merkel Cell Polyomavirus Transformation and Replication
W Liu, J You
Annual Review of Virology 2020
Merkel Cell Polyomavirus Large T Antigen is Dispensable in G2 and M-Phase to Promote Proliferation of Merkel Cell Carcinoma Cells
R Houben, M Ebert, S Hesbacher, T Kervarrec, D Schrama
Viruses 2020
Merkel Cell Polyomavirus Encodes Circular RNAs (circRNAs) Enabling a Dynamic circRNA/microRNA/mRNA Regulatory Network
B Abere, H Zhou, J Li, S Cao, T Toptan, A Grundhoff, N Fischer, PS Moore, Y Chang, VR Racaniello
mBio 2020
Virus-Specific T Cells for the Treatment of Malignancies—Then, Now, and the Future
S Sharma, WK Leung, HE Heslop
Current Stem Cell Reports 2020
DETECTing Merkel Cell Polyomavirus in Merkel Tumors
R Arora, K Gupta, A Vijaykumar, S Krishna
Frontiers in Molecular Biosciences 2020
Tumor Ulceration, Reduced Infiltration of CD8-Lymphocytes, High Neutrophil-to-CD8-Lymphocyte Ratio and Absence of MC Virus are Negative Prognostic Markers for Patients with Merkel Cell Carcinoma
S Naseri, T Steiniche, JB Georgsen, R Thomsen, M Ladekarl, M Heje, TE Damsgaard, ML Bønnelykke-Behrndtz
Cancers 2020

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