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Citations to this article

The liver-specific microRNA miR-122 controls systemic iron homeostasis in mice
Mirco Castoldi, … , Matthias W. Hentze, Martina U. Muckenthaler
Mirco Castoldi, … , Matthias W. Hentze, Martina U. Muckenthaler
Published March 1, 2011
Citation Information: J Clin Invest. 2011;121(4):1386-1396. https://doi.org/10.1172/JCI44883.
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Research Article Hepatology Article has an altmetric score of 7

The liver-specific microRNA miR-122 controls systemic iron homeostasis in mice

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Abstract

Systemic iron homeostasis is mainly controlled by the liver through synthesis of the peptide hormone hepcidin (encoded by Hamp), the key regulator of duodenal iron absorption and macrophage iron release. Here we show that the liver-specific microRNA miR-122 is important for regulating Hamp mRNA expression and tissue iron levels. Efficient and specific depletion of miR-122 by injection of a locked-nucleic-acid–modified (LNA-modified) anti-miR into WT mice caused systemic iron deficiency, characterized by reduced plasma and liver iron levels, mildly impaired hematopoiesis, and increased extramedullary erythropoiesis in the spleen. Moreover, miR-122 inhibition increased the amount of mRNA transcribed by genes that control systemic iron levels, such as hemochromatosis (Hfe), hemojuvelin (Hjv), bone morphogenetic protein receptor type 1A (Bmpr1a), and Hamp. Importantly, miR-122 directly targeted the 3′ untranslated region of 2 mRNAs that encode activators of hepcidin expression, Hfe and Hjv. These data help to explain the increased Hamp mRNA levels and subsequent iron deficiency in mice with reduced miR-122 levels and establish a direct mechanistic link between miR-122 and the regulation of systemic iron metabolism.

Authors

Mirco Castoldi, Maja Vujic Spasic, Sandro Altamura, Joacim Elmén, Morten Lindow, Judit Kiss, Jens Stolte, Richard Sparla, Lorenza A. D’Alessandro, Ursula Klingmüller, Robert E. Fleming, Thomas Longerich, Hermann J. Gröne, Vladimir Benes, Sakari Kauppinen, Matthias W. Hentze, Martina U. Muckenthaler

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2009 Total
Citations: 3 3 7 8 7 10 7 6 7 11 11 12 14 18 4 1 129
Citation information
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Citations to this article in year 2015 (11)

Title and authors Publication Year
Decreased miR122 in hepatocellular carcinoma leads to chemoresistance with increased arginine
Takahiro Kishikawa, Motoyuki Otsuka, Poh Seng Tan, Motoko Ohno, Xiaochen Sun, Takeshi Yoshikawa, Chikako Shibata, Akemi Takata, Kentaro Kojima, Kenji Takehana, Maki Ohishi, Sana Ota, Tomoyuki Noyama, Yuji Kondo, Masaya Sato, Tomoyoshi Soga, Yujin Hoshida, Kazuhiko Koike
Oncotarget 2015
Saturated Fatty Acids Induce Post-transcriptional Regulation of HAMP mRNA via AU-rich Element-binding Protein, Human Antigen R (HuR)
S Lu, JL Mott, DD Harrison-Findik
The Journal of biological chemistry 2015
Hyperosmotic stress activates the expression of members of the miR-15/107 family and induces downregulation of anti-apoptotic genes in rat liver
D Santosa, M Castoldi, M Paluschinski, A Sommerfeld, D Häussinger
Scientific Reports 2015
Hepatitis C Virus RNA Functionally Sequesters miR-122
JM Luna, TK Scheel, T Danino, KS Shaw, A Mele, JJ Fak, E Nishiuchi, CN Takacs, MT Catanese, YP de Jong, IM Jacobson, CM Rice, RB Darnell
Cell 2015
Demonstration of the Presence of the “Deleted” MIR122 Gene in HepG2 Cells
IA Hamad, Y Fei, AZ Kalea, D Yin, AJ Smith, J Palmen, SE Humphries, PJ Talmud, AP Walker, S Pfeffer
PloS one 2015
Identification of cytokine-induced modulation of microRNA expression and secretion as measured by a novel microRNA specific qPCR assay
V Benes, P Collier, C Kordes, J Stolte, T Rausch, MU Muckentaler, D Häussinger, M Castoldi
Scientific Reports 2015
Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division
M Sidova, R Sindelka, M Castoldi, V Benes, M Kubista
Scientific Reports 2015
Hepatitis B virus and microRNAs: Complex interactions affecting hepatitis B virus replication and hepatitis B virus-associated diseases
J Lamontagne
World journal of gastroenterology : WJG 2015
miR-20a regulates expression of the iron exporter ferroportin in lung cancer
KR Babu, MU Muckenthaler
Journal of Molecular Medicine 2015
Elevating microRNA-122 in blood improves outcomes after temporary middle cerebral artery occlusion in rats
DZ Liu, GC Jickling, BP Ander, H Hull, X Zhan, C Cox, N Shroff, C Dykstra-Aiello, B Stamova, FR Sharp
Journal of Cerebral Blood Flow & Metabolism 2015
miR-122 is a Unique Molecule with Great Potential in Diagnosis, Prognosis of Liver Disease, and Therapy Both as miRNA Mimic and Antimir
Thakral S, Ghoshal K
Current gene therapy 2015

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