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Citations to this article

Increased NOS2 predicts poor survival in estrogen receptor–negative breast cancer patients
Sharon A. Glynn, … , Robert M. Stephens, Stefan Ambs
Sharon A. Glynn, … , Robert M. Stephens, Stefan Ambs
Published October 18, 2010
Citation Information: J Clin Invest. 2010;120(11):3843-3854. https://doi.org/10.1172/JCI42059.
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Research Article Oncology Article has an altmetric score of 3

Increased NOS2 predicts poor survival in estrogen receptor–negative breast cancer patients

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Abstract

Inducible nitric oxide synthase (NOS2) is involved in wound healing, angiogenesis, and carcinogenesis. NOS2 upregulation and increased nitric oxide (NO) production affect the redox state of cells and can induce protein, lipid, and DNA modifications. To investigate whether NOS2 levels influence survival of breast cancer patients, we examined NOS2 expression and its association with tumor markers and survival in 248 breast tumors. In multivariable survival analysis, increased NOS2 predicted inferior survival in women with estrogen receptor α–negative (ER-negative) tumors. Microdissected tumor epithelium from ER-negative tumors with high NOS2 had increased IL-8 and a gene expression signature characteristic of basal-like breast cancer with poor prognosis. In cell culture, NO only induced selected signature genes in ER-negative breast cancer cells. ER transgene expression in ER-negative cells inhibited NO-induced upregulation of the stem cell marker CD44 and other proteins encoded by signature genes, but not of IL-8. Exposure to NO also enhanced cell motility and invasion of ER-negative cells. Last, pathway analysis linked the tumor NOS2 gene signature to c-Myc activation. Thus, NOS2 is associated with a basal-like transcription pattern and poor survival of ER-negative patients.

Authors

Sharon A. Glynn, Brenda J. Boersma, Tiffany H. Dorsey, Ming Yi, Harris G. Yfantis, Lisa A. Ridnour, Damali N. Martin, Christopher H. Switzer, Robert S. Hudson, David A. Wink, Dong H. Lee, Robert M. Stephens, Stefan Ambs

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2009 Total
Citations: 6 12 14 10 8 9 4 5 8 12 15 8 11 16 5 1 144
Citation information
This citation data is accumulated from CrossRef, which receives citation information from participating publishers, including this journal. Not all publishers participate in CrossRef, so this information is not comprehensive. Additionally, data may not reflect the most current citations to this article, and the data may differ from citation information available from other sources (for example, Google Scholar, Web of Science, and Scopus).

Citations to this article in year 2012 (16)

Title and authors Publication Year
S-nitrosylation of EGFR and Src activates an oncogenic signaling network in human basal-like breast cancer
CH Switzer, SA Glynn, RY Cheng, LA Ridnour, JE Green, S Ambs, DA Wink
Molecular cancer research : MCR 2012
Dithiolethiones Inhibit NF-κB Activity via Covalent Modification in Human Estrogen Receptor–Negative Breast Cancer
CH Switzer, RY Cheng, LA Ridnour, MC Murray, V Tazzari, A Sparatore, PD Soldato, HB Hines, SA Glynn, S Ambs, DA Wink
Cancer research 2012
Molecular Mechanisms of Nitric Oxide in Cancer Progression, Signal Transduction, and Metabolism
V Somasundaram, D Basudhar, G Bharadwaj, JH No, LA Ridnour, RY Cheng, M Fujita, DD Thomas, SK Anderson, DW McVicar, DA Wink
Antioxidants & Redox Signaling 2012
Immunotherapeutic Potential of Anti-Human Endogenous Retrovirus-K Envelope Protein Antibodies in Targeting Breast Tumors
F Wang-Johanning, K Rycaj, JB Plummer, M Li, B Yin, K Frerich, JG Garza, J Shen, K Lin, P Yan, SA Glynn, TH Dorsey, KK Hunt, S Ambs, GL Johanning
JNCI Journal of the National Cancer Institute 2012
Nitric Oxide Synthase and Breast Cancer: Role of TIMP-1 in NO-mediated Akt Activation
LA Ridnour, KM Barasch, AN Windhausen, TH Dorsey, MM Lizardo, HG Yfantis, DH Lee, CH Switzer, RY Cheng, JL Heinecke, E Brueggemann, HB Hines, C Khanna, SA Glynn, S Ambs, DA Wink
PloS one 2012
Relationship between Tumor DNA Methylation Status and Patient Characteristics in African-American and European-American Women with Breast Cancer
S Wang, TH Dorsey, A Terunuma, RA Kittles, S Ambs, B Kwabi-Addo
PloS one 2012
Ets-1 is a transcriptional mediator of oncogenic nitric oxide signaling in estrogen receptor-negative breast cancer
CH Switzer, RY Cheng, LA Ridnour, SA Glynn, S Ambs, DA Wink
Breast Cancer Research 2012
Macrophage migration inhibitory factor induces epithelial to mesenchymal transition, enhances tumor aggressiveness and predicts clinical outcome in resected pancreatic ductal adenocarcinoma
N Funamizu, C Hu, C Lacy, A Schetter, G Zhang, P He, J Gaedcke, MB Ghadimi, T Ried, HG Yfantis, DH Lee, J Subleski, T Chan, JM Weiss, TC Back, K Yanaga, N Hanna, HR Alexander, A Maitra, SP Hussain
International Journal of Cancer 2012
Identification of mammary epithelial cells subject to chronic oxidative stress in mammary epithelium of young women and teenagers living in USA: Implication for breast carcinogenesis
J Weisz, DA Shearer, E Murata, SD Patrick, B Han, A Berg, GA Clawson
Cancer biology & therapy 2012
S-Nitrosation Mediates Multiple Pathways That Lead to Tumor Progression in Estrogen Receptor-Negative Breast Cancer
CH Switzer, LA Ridnour, R Cheng, J Heinecke, A Burke, S Glynn, S Ambs, DA Wink
Forum on immunopathological diseases and therapeutics 2012
The roles of beta-adrenergic receptors in tumorigenesis and the possible use of beta-adrenergic blockers for cancer treatment: possible genetic and cell-signaling mechanisms
KV Lu'o'ng, LT Nguyễn
Cancer management and research 2012
Redox regulation of epidermal growth factor receptor signaling through cysteine oxidation
TH Truong, KS Carroll
Biochemistry 2012
S-nitrosylation of Ras in breast cancer
HE Marshall, MW Foster
Breast Cancer Research 2012
Molecular Pathways: Toll-like Receptors in the Tumor Microenvironment--Poor Prognosis or New Therapeutic Opportunity
LA Ridnour, RY Cheng, CH Switzer, JL Heinecke, S Ambs, S Glynn, HA Young, G Trinchieri, DA Wink
Clinical cancer research 2012
Part II. Mitochondrial mutational status of high nitric oxide adapted cell line BT-20 (BT-20-HNO) as it relates to human primary breast tumors
HD Vitto, BS Mendonça, KM Elseth, BJ Vesper, EA Portari, CV Gallo, WA Paradise, FD Rumjanek, JA Radosevich
Tumor Biology 2012
Molecular subtype is determinant on inflammatory status and immunological profile from invasive breast cancer patients.
Herrera AC, Panis C, Victorino VJ, Campos FC, Colado-Simão AN, Cecchini AL, Cecchini R
Cancer Immunology, Immunotherapy 2012

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