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Citations to this article

Autophagy regulates adipose mass and differentiation in mice
Rajat Singh, … , Gary J. Schwartz, Mark J. Czaja
Rajat Singh, … , Gary J. Schwartz, Mark J. Czaja
Published October 12, 2009
Citation Information: J Clin Invest. 2009;119(11):3329-3339. https://doi.org/10.1172/JCI39228.
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Research Article Metabolism Article has an altmetric score of 1

Autophagy regulates adipose mass and differentiation in mice

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Abstract

The relative balance between the quantity of white and brown adipose tissue can profoundly affect lipid storage and whole-body energy homeostasis. However, the mechanisms regulating the formation, expansion, and interconversion of these 2 distinct types of fat remain unknown. Recently, the lysosomal degradative pathway of macroautophagy has been identified as a regulator of cellular differentiation, suggesting that autophagy may modulate this process in adipocytes. The function of autophagy in adipose differentiation was therefore examined in the current study by genetic inhibition of the critical macroautophagy gene autophagy-related 7 (Atg7). Knockdown of Atg7 in 3T3-L1 preadipocytes inhibited lipid accumulation and decreased protein levels of adipocyte differentiation factors. Knockdown of Atg5 or pharmacological inhibition of autophagy or lysosome function also had similar effects. An adipocyte-specific mouse knockout of Atg7 generated lean mice with decreased white adipose mass and enhanced insulin sensitivity. White adipose tissue in knockout mice had increased features of brown adipocytes, which, along with an increase in normal brown adipose tissue, led to an elevated rate of fatty acid, β-oxidation, and a lean body mass. Autophagy therefore functions to regulate body lipid accumulation by controlling adipocyte differentiation and determining the balance between white and brown fat.

Authors

Rajat Singh, Youqing Xiang, Yongjun Wang, Kiran Baikati, Ana Maria Cuervo, Yen K. Luu, Yan Tang, Jeffrey E. Pessin, Gary J. Schwartz, Mark J. Czaja

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 Total
Citations: 4 17 20 28 38 30 36 35 22 21 25 27 40 31 21 18 1 414
Citation information
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Citations to this article in year 2011 (21)

Title and authors Publication Year
Ablation of a galectin preferentially expressed in adipocytes increases lipolysis, reduces adiposity, and improves insulin sensitivity in mice
RY Yang, L Yu, JL Graham, DK Hsu, KC Lloyd, PJ Havel, FT Liu
Proceedings of the National Academy of Sciences 2011
Comprehensive Physiology
R Terjung
Comprehensive Physiology 2011
Functions of Autophagy in Hepatic and Pancreatic Physiology and Disease
MJ Czaja
Gastroenterology 2011
Autophagy in nonalcoholic steatohepatitis
M Amir, MJ Czaja
Expert Review of Gastroenterology & Hepatology 2011
Autophagic Degradation of Mitochondria in White Adipose Tissue Differentiation
SJ Goldman, Y Zhang, S Jin
Antioxidants & Redox Signaling 2011
Autophagy in Tumorigenesis and Energy Metabolism: Friend by Day, Foe by Night
R Mathew, E White
Current Opinion in Genetics & Development 2011
Autophagy and disease: always two sides to a problem
S Sridhar, Y Botbol, F Macian, AM Cuervo
The Journal of Pathology 2011
Role of autophagy in cancer prevention
HY Chen, E White
Cancer prevention research (Philadelphia, Pa.) 2011
Autophagy regulates myeloid cell differentiation by p62/SQSTM1-mediated degradation of PML-RARα oncoprotein
Z Wang, L Cao, R Kang, M Yang, L Liu, Y Zhao, Y Yu, M Xie, X Yin, KM Livesey, D Tang
Autophagy 2011
Temporal orchestration of circadian autophagy rhythm by C/EBPβ
D Ma, S Panda, JD Lin
The EMBO Journal 2011
Systems biology of the autophagy-lysosomal pathway
AG Jegga, L Schneider, X Ouyang, J Zhang
Autophagy 2011
Autophagy Regulates Cholesterol Efflux from Macrophage Foam Cells via Lysosomal Acid Lipase
M Ouimet, V Franklin, E Mak, X Liao, I Tabas, YL Marcel
Cell Metabolism 2011
Prolonged QT interval and lipid alterations beyond β-oxidation in very long-chain acyl-CoA dehydrogenase null mouse hearts
R Gélinas, J Thompson-Legault, B Bouchard, C Daneault, A Mansour, MA Gillis, G Charron, V Gavino, F Labarthe, CD Rosiers
American journal of physiology. Heart and circulatory physiology 2011
Autophagy in the Cellular Energetic Balance
R Singh, AM Cuervo
Cell Metabolism 2011
A lipid-droplet-targeted O-GlcNAcase isoform is a key regulator of the proteasome
CN Keembiyehetty, A Krzeslak, DC Love, JA Hanover
Journal of cell science 2011
REGULATION OF LIPID DROPLETS BY AUTOPHAGY
H Dong, MJ Czaja
Trends in endocrinology and metabolism: TEM 2011
Autophagy in hypothalamic AgRP neurons regulates food intake and energy balance
S Kaushik, JA Rodriguez-Navarro, E Arias, R Kiffin, S Sahu, GJ Schwartz, AM Cuervo, R Singh
Cell Metabolism 2011
Defective Hypothalamic Autophagy Directs the Central Pathogenesis of Obesity via the IκB Kinase β (IKKβ)/NF-κB Pathway*
Q Meng, D Cai
The Journal of biological chemistry 2011
Osteoclast precursor differentiation by MCPIP via oxidative stress, endoplasmic reticulum stress, and autophagy
K Wang, J Niu, H Kim, PE Kolattukudy
Journal of Molecular Cell Biology 2011
A differential autophagic response to hyperglycemia in the developing murine embryo
KL Adastra, MM Chi, JK Riley, KH Moley
Reproduction 2011
Differential roles of unsaturated and saturated fatty acids on autophagy and apoptosis in hepatocytes
S Mei, HM Ni, S Manley, A Bockus, KM Kassel, JP Luyendyk, BL Copple, WX Ding
The Journal of pharmacology and experimental therapeutics 2011

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