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Citations to this article

Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell–derived lin–CD34+CD43+CD45+ progenitors
Kyung-Dal Choi, … , Maxim A. Vodyanik, Igor I. Slukvin
Kyung-Dal Choi, … , Maxim A. Vodyanik, Igor I. Slukvin
Published August 10, 2009
Citation Information: J Clin Invest. 2009;119(9):2818-2829. https://doi.org/10.1172/JCI38591.
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Technical Advance Hematology Article has an altmetric score of 22

Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell–derived lin–CD34+CD43+CD45+ progenitors

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Abstract

Basic research into human mature myelomonocytic cell function, myeloid lineage diversification and leukemic transformation, and assessment of myelotoxicity in preclinical drug development requires a constant supply of donor blood or bone marrow samples and laborious purification of mature myeloid cells or progenitors, which are present in very small quantities. To overcome these limitations, we have developed a protocol for efficient generation of neutrophils, eosinophils, macrophages, osteoclasts, DCs, and Langerhans cells from human embryonic stem cells (hESCs). As a first step, we generated lin–CD34+CD43+CD45+ hematopoietic cells highly enriched in myeloid progenitors through coculture of hESCs with OP9 feeder cells. After expansion in the presence of GM-CSF, these cells were directly differentiated with specific cytokine combinations toward mature cells of particular types. Morphologic, phenotypic, molecular, and functional analyses revealed that hESC-derived myelomonocytic cells were comparable to their corresponding somatic counterparts. In addition, we demonstrated that a similar protocol could be used to generate myelomonocytic cells from induced pluripotent stem cells (iPSCs). This technology offers an opportunity to generate large numbers of patient-specific myelomonocytic cells for in vitro studies of human disease mechanisms as well as for drug screening.

Authors

Kyung-Dal Choi, Maxim A. Vodyanik, Igor I. Slukvin

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 Total
Citations: 1 4 8 5 9 11 7 8 8 11 9 8 7 8 7 8 1 120
Citation information
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Citations to this article in year 2012 (8)

Title and authors Publication Year
Mesodermal and Hematopoietic Differentiation from ES and iPS Cells
T Inoue-Yokoo, K Tani, D Sugiyama
Stem Cell Reviews and Reports 2012
Identification of the Hemogenic Endothelial Progenitor and Its Direct Precursor in Human Pluripotent Stem Cell Differentiation Cultures
KD Choi, MA Vodyanik, PP Togarrati, K Suknuntha, A Kumar, F Samarjeet, MD Probasco, S Tian, R Stewart, JA Thomson, II Slukvin
Cell Reports 2012
Generation of red blood cells from human embryonic/induced pluripotent stem cells for blood transfusion
Y Ebihara, F Ma, K Tsuji
International Journal of Hematology 2012
Generation of mature hematopoietic cells from human pluripotent stem cells
PP Togarrati, K Suknuntha
International Journal of Hematology 2012
Pluripotent Stem Cells: Immune to the Immune System?
JI Pearl, LS Kean, MM Davis, JC Wu
Science Translational Medicine 2012
In vivo generation of transplantable human hematopoietic cells from induced pluripotent stem cells
G Amabile, RS Welner, C Nombela-Arrieta, AM D'Alise, AD Ruscio, AK Ebralidze, Y Kraytsberg, M Ye, O Kocher, DS Neuberg, K Khrapko, LE Silberstein, DG Tenen
Blood 2012
Novel, high-yield red blood cell production methods from CD34-positive cells derived from human embryonic stem, yolk sac, fetal liver, cord blood, and peripheral blood
E Olivier, C Qiu, EE Bouhassira
Stem Cells Translational Medicine 2012
Generation of Induced Pluripotent Stem Cells from Mouse Cancer Cells
FK Lin, YL Chui
Cancer Biotherapy & Radiopharmaceuticals 2012

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