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Citations to this article

Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell–derived lin–CD34+CD43+CD45+ progenitors
Kyung-Dal Choi, … , Maxim A. Vodyanik, Igor I. Slukvin
Kyung-Dal Choi, … , Maxim A. Vodyanik, Igor I. Slukvin
Published August 10, 2009
Citation Information: J Clin Invest. 2009;119(9):2818-2829. https://doi.org/10.1172/JCI38591.
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Technical Advance Hematology Article has an altmetric score of 22

Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell–derived lin–CD34+CD43+CD45+ progenitors

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Abstract

Basic research into human mature myelomonocytic cell function, myeloid lineage diversification and leukemic transformation, and assessment of myelotoxicity in preclinical drug development requires a constant supply of donor blood or bone marrow samples and laborious purification of mature myeloid cells or progenitors, which are present in very small quantities. To overcome these limitations, we have developed a protocol for efficient generation of neutrophils, eosinophils, macrophages, osteoclasts, DCs, and Langerhans cells from human embryonic stem cells (hESCs). As a first step, we generated lin–CD34+CD43+CD45+ hematopoietic cells highly enriched in myeloid progenitors through coculture of hESCs with OP9 feeder cells. After expansion in the presence of GM-CSF, these cells were directly differentiated with specific cytokine combinations toward mature cells of particular types. Morphologic, phenotypic, molecular, and functional analyses revealed that hESC-derived myelomonocytic cells were comparable to their corresponding somatic counterparts. In addition, we demonstrated that a similar protocol could be used to generate myelomonocytic cells from induced pluripotent stem cells (iPSCs). This technology offers an opportunity to generate large numbers of patient-specific myelomonocytic cells for in vitro studies of human disease mechanisms as well as for drug screening.

Authors

Kyung-Dal Choi, Maxim A. Vodyanik, Igor I. Slukvin

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Total citations by year

Year: 2025 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 Total
Citations: 1 4 8 5 9 11 7 8 8 11 9 8 7 8 7 8 1 120
Citation information
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Citations to this article in year 2010 (8)

Title and authors Publication Year
Human pluripotent stem cells produce natural killer cells that mediate anti-HIV-1 activity by utilizing diverse cellular mechanisms
Z Ni, DA Knorr, CL Clouser, MK Hexum, P Southern, LM Mansky, IH Park, DS Kaufman
Journal of virology 2010
Pluripotent stem cell-derived natural killer cells for cancer therapy
DA Knorr, DS Kaufman
Translational research : the journal of laboratory and clinical medicine 2010
The challenge of immunogenicity in the quest for induced pluripotency
PJ Fairchild
Nature Reviews Immunology 2010
Generation of mature blood cells from pluripotent stem cells
II Slukvin
Haematologica 2010
Red blood cell generation from human induced pluripotent stem cells: perspectives for transfusion medicine
H Lapillonne, L Kobari, C Mazurier, P Tropel, MC Giarratana, I Zanella-Cleon, L Kiger, M Wattenhofer-Donzé, H Puccio, N Hebert, A Francina, G Andreu, S Viville, L Douay
Haematologica 2010
Directed differentiation of hematopoietic precursors and functional osteoclasts from human ES and iPS cells
AE Grigoriadis, M Kennedy, A Bozec, F Brunton, G Stenbeck, IH Park, EF Wagner, GM Keller
Blood 2010
Potential of human induced pluripotent stem cells derived from blood and other postnatal cell types
Z Ye, L Cheng
Regenerative Medicine 2010
Pluripotent stem cells as source of dendritic cells for immune therapy
S Senju, S Hirata, Y Motomura, D Fukuma, Y Matsunaga, S Fukushima, H Matsuyoshi, Y Nishimura
International Journal of Hematology 2010

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