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Citations to this article

Local production of angiotensin II in the subfornical organ causes elevated drinking
Koji Sakai, … , Robin L. Davisson, Curt D. Sigmund
Koji Sakai, … , Robin L. Davisson, Curt D. Sigmund
Published April 2, 2007
Citation Information: J Clin Invest. 2007;117(4):1088-1095. https://doi.org/10.1172/JCI31242.
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Research Article Article has an altmetric score of 3

Local production of angiotensin II in the subfornical organ causes elevated drinking

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Abstract

The mechanism controlling cell-specific Ang II production in the brain remains unclear despite evidence supporting neuron-specific renin and glial- and neuronal-specific angiotensinogen (AGT) expression. We generated double-transgenic mice expressing human renin (hREN) from a neuron-specific promoter and human AGT (hAGT) from its own promoter (SRA mice) to emulate this expression. SRA mice exhibited an increase in water and salt intake and urinary volume, which were significantly reduced after chronic intracerebroventricular delivery of losartan. Ang II–like immunoreactivity was markedly increased in the subfornical organ (SFO). To further evaluate the physiological importance of de novo Ang II production specifically in the SFO, we utilized a transgenic mouse model expressing a floxed version of hAGT (hAGTflox), so that deletions could be induced with Cre recombinase. We targeted SFO-specific ablation of hAGTflox by microinjection of an adenovirus encoding Cre recombinase (AdCre). SRAflox mice exhibited a marked increase in drinking at baseline and a significant decrease in water intake after administration of AdCre/adenovirus encoding enhanced GFP (AdCre/AdEGFP), but not after administration of AdEGFP alone. This decrease only occurred when Cre recombinase correctly targeted the SFO and correlated with a loss of hAGT and angiotensin peptide immunostaining in the SFO. These data provide strong genetic evidence implicating de novo synthesis of Ang II in the SFO as an integral player in fluid homeostasis.

Authors

Koji Sakai, Khristofor Agassandian, Satoshi Morimoto, Puspha Sinnayah, Martin D. Cassell, Robin L. Davisson, Curt D. Sigmund

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Total citations by year

Year: 2024 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 Total
Citations: 6 1 5 2 4 2 4 3 5 5 7 4 4 4 3 4 6 2 71
Citation information
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Citations to this article in year 2009 (4)

Title and authors Publication Year
Genetic silencing of Nox2 and Nox4 reveals differential roles of these NADPH oxidase homologues in the vasopressor and dipsogenic effects of brain angiotensin II
JR Peterson, MA Burmeister, X Tian, Y Zhou, MR Guruju, JA Stupinski, RV Sharma, RL Davisson
Hypertension 2009
Pulmonary surfactant: an immunological perspective
ZC Chroneos, Z Sever-Chroneos, VL Shepherd
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2009
Systemic candesartan reduces brain angiotensin II via downregulation of brain renin-angiotensin system
N Pelisch, N Hosomi, M Ueno, H Masugata, K Murao, H Hitomi, D Nakano, H Kobori, A Nishiyama, M Kohno
Hypertension research : official journal of the Japanese Society of Hypertension 2009
Chronic immunoneutralization of brain angiotensin-(1-12) lowers blood pressure in transgenic (mRen2)27 hypertensive rats
K Isa, MA García-Espinosa, AC Arnold, NT Pirro, EN Tommasi, D Ganten, MC Chappell, CM Ferrario, DI Diz
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 2009

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